Toxoplasma gondii triggers secretion of interleukin-12 but low level Of interleukin-10 from the THP-1 human monocytic cell line

被引：23

作者：

Aldebert, Delphine ^{[1
]}

Durand, Francois ^{[1
]}

Mercier, Corinne ^{[1
]}

Brenier-Pinchart, Marie-Pierre ^{[1
]}

Cesbron-Delauw, Marie-France ^{[1
]}

Pelloux, Herve ^{[1
]}

机构：

[1] Univ Grenoble 1, CNRS, UMR 5163, Lab Adaptat & Pathogenie Microorganismes,Inst J R, F-38042 Grenoble 9, France

来源：

CYTOKINE | 2007年 / 37卷 / 03期

关键词：

interleukin-12; interleukin-10; Toxoplasma gondii; THP-1; human;

D O I：

10.1016/j.cyto.2007.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies using both in vitro and in vivo mouse models have demonstrated that a subtle balance between pro- and anti-inflammatory cytokines, among.which interleukin- 12 (IL-12) and interleukin-10 (IL-10), respectively is crucial to control Toxoplasma infection. However, the few studies performed with human cell lines highlighted important host-related differences in the immune response to Toxoplasma gondii. The goal of our work was thus to study the production of both IL-12 and IL-10 by the THP-1 human monocytic cell line in response to Toxoplasma. We demonstrated that infection by live parasites (RH strain) triggers secretion of IL-12, but low level of IL-10. IL-12 secretion appeared within 8 h, up to 48 h. We also showed that infection by live parasites is not mandatory since heat-killed parasites, crude tachyzoite lysate as well as excreted/secreted antigens induced significant, yet reduced production of IL-12. (c) 2007 Elsevier Ltd. All rights reserved.

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页码：206 / 211

页数：6

相关论文

共 30 条

[1] Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells [J].

Alberti, J ;

Valenzuela, JG ;

Carruthers, VB ;

Hieny, S ;

Andersen, J ;

Charest, H ;

Sousa, CRE ;

Fairlamb, A ;

Ribeiro, JM ;

Sher, A .

NATURE IMMUNOLOGY, 2003, 4 (05) :485-490

[2] Lipoxin-mediated inhibition of IL-12 production by DCs: a mechanism for regulation of microbial immunity [J].

Aliberti, J ;

Hieny, S ;

Sousa, CRE ;

Serhan, CN ;

Sher, A .

NATURE IMMUNOLOGY, 2002, 3 (01) :76-82

[3] Toxoplasma gondii-derived heat shock protein 70 stimulates maturation of murine bone marrow-derived dendritic cells via Toll-like receptor 4 [J].

Aosai, F ;

Pena, MSR ;

Mun, HS ;

Fang, H ;

Mitsunaga, T ;

Norose, K ;

Kang, HK ;

Bae, YS ;

Yano, A .

CELL STRESS & CHAPERONES, 2006, 11 (01) :13-22

[4] Involvement of tumor necrosis factor-α during infection of human monocytic cells by Toxoplasma gondii [J].

Belloni, A ;

Aubert, D ;

Marin, JEG ;

Le Naour, R ;

Bonhomme, A ;

Guenounou, M ;

Pinon, JM .

PARASITOLOGY RESEARCH, 2000, 86 (05) :406-412

[5]

Bliss SK, 1999, J IMMUNOL, V162, P7369

[6] The Toxoplasma surface protein SAG1 triggers efficient in vitro secretion of chemokine ligand 2 (CCL2) from human fibroblasts [J].

Brenier-Pinchart, MP ;

Villena, I ;

Mercier, C ;

Durand, F ;

Simon, J ;

Cesbron-Delauw, MF ;

Pelloux, H .

MICROBES AND INFECTION, 2006, 8 (01) :254-261

[7] Mechanism of entry determines the ability of Toxoplasma gondii to inhibit macrophage proinflammatory cytokine production [J].

Butcher, BA ;

Denkers, EY .

INFECTION AND IMMUNITY, 2002, 70 (09) :5216-5224

[8] Toxoplasma gondii tachyzoites inhibit proinflammatory cytokine induction in infected macrophages by preventing nuclear translocation of the transcription factor NF-κB [J].

Butcher, BA ;

Kim, L ;

Johnson, PF ;

Denkers, EY .

JOURNAL OF IMMUNOLOGY, 2001, 167 (04) :2193-2201

[9] INDUCTION OF A PROTECTIVE ANTIBODY-DEPENDENT RESPONSE AGAINST TOXOPLASMOSIS BY INVITRO EXCRETED SECRETED ANTIGENS FROM TACHYZOITES OF TOXOPLASMA-GONDII [J].

DARCY, F ;

DESLEE, D ;

SANTORO, F ;

CHARIF, H ;

AURIAULT, C ;

DECOSTER, A ;

DUQUESNE, V ;

CAPRON, A .

PARASITE IMMUNOLOGY, 1988, 10 (05) :553-567

[10] Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection [J].

Denkers, EY ;

Gazzinelli, RT .

CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (04) :569-+