Effects of phosphorothioate oligodeoxyribonucleotide and oligoribonucleotides on human complement and coagulation

被引：14

作者：

Kandimalla, ER

Shaw, DR

Agrawal, S

机构：

[1] Hybridon Inc, Cambridge, MA 02139 USA

[2] Univ Alabama Birmingham, Dept Med, Div Hematol & Oncol, Birmingham, AL 35294 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1998年 / 8卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(98)00368-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We have synthesized and studied the effects of phosphorothioate (PS) oligodeoxyribonucleotide (DNA) and oligoribonucleotides (RNA, 2'-O-methyl-RNA and 2'-5'-RNA) on complement activation and prolongation of activated partial thromboplastin time (aPTT) in vitro. These results suggest that a PS-DNA prolongs aPTT, and inhibits complement lysis more than do the PS-RNA analogs. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：2103 / 2108

页数：6

相关论文

共 24 条

[1] SYNTHESIS AND ANTI-HIV ACTIVITY OF OLIGORIBONUCLEOTIDES AND THEIR PHOSPHOROTHIOATE ANALOGS [J].

AGRAWAL, S ;

TANG, JY ;

SUN, D ;

SARIN, PS ;

ZAMECNIK, PC .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1992, 660 :2-10

[2] EFFICIENT SYNTHESIS OF OLIGORIBONUCLEOTIDE AND ITS PHOSPHOROTHIOATE ANALOG USING H-PHOSPHONATE APPROACH [J].

AGRAWAL, S ;

TANG, JY .

TETRAHEDRON LETTERS, 1990, 31 (52) :7541-7544

[3] Mixed-backbone oligonucleotides as second generation antisense oligonucleotides: In vitro and in vivo studies [J].

Agrawal, S ;

Jiang, ZW ;

Zhao, QY ;

Shaw, D ;

Cai, QY ;

Roskey, A ;

Channavajjala, L ;

Saxinger, C ;

Zhang, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2620-2625

[4] Antisense oligonucleotides: Towards clinical trials [J].

Agrawal, S .

TRENDS IN BIOTECHNOLOGY, 1996, 14 (10) :376-387

[5] Novel enzymatic and immunological responses to oligonucleotides [J].

Agrawal, S ;

Rustagi, PK ;

Shaw, DR .

TOXICOLOGY LETTERS, 1995, 82-3 :431-434

[6]

AGRAWAL S, 1997, ANTISENSE OLIGODEOXY, P57

[7]

Agrawal S., 1996, ANTISENSE THERAPEUTI

[8]

Bayever E, 1993, Antisense Res Dev, V3, P383

[9] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[10] A PHARMACOKINETIC EVALUATION OF C-14-LABELED AFOVIRSEN SODIUM IN PATIENTS WITH GENITAL WARTS [J].

CROOKE, ST ;

GRILLONE, LR ;

TENDOLKAR, A ;

GARRETT, A ;

FRATKIN, MJ ;

LEEDS, J ;

BARR, WH .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 1994, 56 (06) :641-646