Two-photon autofluorescence spectroscopy and second-harmonic generation of epithelial tissue

被引：46

作者：

Wu, YC ^{[1
]}

Qu, JNY ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Elect & Elect Engn, Kowloon, Hong Kong, Peoples R China

来源：

OPTICS LETTERS | 2005年 / 30卷 / 22期

关键词：

D O I：

10.1364/OL.30.003045

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A spectroscopy system is developed for studying the two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) of epithelial tissue in backscattering geometry. Our findings show that TPEF signals from epithelial and underlying stromal layers exhibit different spectral characteristics, providing information on the biomorphology and biochemistry of tissue. The SHG signal serves as a sensitive indicator of collagen to separate the epithelial layer from underlying stroma. The polarization dependence of the SHG signal reveals a well-ordered orientation of collagen fibers in the stromal layer. The results demonstrate the potential of depth-resolved TPEF and SHG in determining the pathology of epithelial tissue. (c) 2005 Optical Society of America.

引用

页码：3045 / 3047

页数：3

共 9 条

[1] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[2] Second-harmonic tomography of tissues [J].

Guo, YC ;

Ho, PP ;

Savage, H ;

Harris, D ;

Sacks, P ;

Schantz, S ;

Liu, F ;

Zhadin, N ;

Alfano, RR .

OPTICS LETTERS, 1997, 22 (17) :1323-1325

[3] Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein [J].

Huang, SH ;

Heikal, AA ;

Webb, WW .

BIOPHYSICAL JOURNAL, 2002, 82 (05) :2811-2825

[4] Fluorescence spectroscopy of neoplastic and non-neoplastic tissues [J].

Ramanujam, N .

NEOPLASIA, 2000, 2 (1-2) :89-117

[5] Polarization-dependent optical second-harmonic imaging of a rat-tail tendon [J].

Stoller, P ;

Kim, BM ;

Rubenchik, AM ;

Reiser, KM ;

Da Silva, LB .

JOURNAL OF BIOMEDICAL OPTICS, 2002, 7 (02) :205-214

[6] Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue [J].

Wu, YC ;

Xi, P ;

Qu, JNY ;

Cheung, TH ;

Yu, MY .

OPTICS EXPRESS, 2005, 13 (02) :382-388

[7] Depth-resolved fluorescence spectroscopy reveals layered structure of tissue [J].

Wu, YC ;

Xi, P ;

Qu, JAY ;

Cheung, TH ;

Yu, MY .

OPTICS EXPRESS, 2004, 12 (14) :3218-3223

[8] Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry [J].

Yasui, T ;

Tohno, Y ;

Araki, T .

JOURNAL OF BIOMEDICAL OPTICS, 2004, 9 (02) :259-264

[9] Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence [J].

Yeh, AT ;

Nassif, N ;

Zoumi, A ;

Tromberg, BJ .

OPTICS LETTERS, 2002, 27 (23) :2082-2084

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