Evidence for acquisition of Legionella type IV secretion substrates via interdomain horizontal gene transfer

被引:219
作者
de Felipe, KS
Pampou, S
Jovanovic, OS
Pericone, CD
Ye, SF
Kalachikov, S
Shuman, HA
机构
[1] Columbia Univ, Med Ctr, Dept Microbiol, New York, NY 10032 USA
[2] Columbia Univ, Med Ctr, Integrated Program Cellular Mol & Biophys Studies, New York, NY 10032 USA
[3] Columbia Univ, Med Ctr, Columbia Genome Ctr, New York, NY 10032 USA
关键词
D O I
10.1128/JB.187.22.7716-7726.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Intracellular pathogens exploit host cell functions to create a replication niche inside eukaryotic cells. The causative agent of Legionnaires' disease, the gamma-proteobacterium Legionella pneumophila, resides and replicates within a modified vacuole of protozoan and mammalian cells. L. pneumophila translocates effector proteins into host cells through the Icm-Dot complex, a specialized type IVB secretion system that is required for intracellular growth. To find out if some effector proteins may have been acquired through interdomain horizontal gene transfer (HGT), we performed a bioinformatic screen that searched for eukaryotic motifs in all open reading frames of the L. pneumophila Philadelphia-1 genome. We found 44 uncharacterized genes with many distinct eukaryotic motifs. Most of these genes contain G+C biases compared to other L. pneumophila genes, supporting the theory that they were acquired through HGT. Furthermore, we found that several of them are expressed and up-regulated in stationary phase in an RpoS-dependent manner. In addition, at least seven of these gene products are translocated into host cells via the Icm-Dot complex, confirming their role in the intracellular environment. Reminiscent of the case with most Icm-Dot substrates, most of the strains containing mutations in these genes grew comparably to the parent strain intracellularly. Our findings suggest that in L. pneumophila, interdomain HGT may have been a major mechanism for the acquisition of determinants of infection.
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页码:7716 / 7726
页数:11
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