Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

被引：2

作者：

Carvalho, AL

Dias, JM

Sanz, L

Romero, A

Calvete, JJ

Romao, MJ ^{[1
]}

机构：

[1] Univ Nova Lisboa, Fac Ciencias & Tecnol, Ctr Quim Fina & Biotecnol, Dept Quim, P-2829516 Monte De Caparica, Portugal

[2] CSIC, Inst Biomed Valencia, E-46010 Valencia, Spain

[3] CSIC, Ctr Invest Biol, E-28006 Madrid, Spain

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901009805

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purired from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Angstrom resolution were collected at the ESRF synchrotron-radiation source.

引用

页码：1180 / 1183

页数：4

共 31 条

[1] Methods used in the structure determination of bovine mitochondrial F-1 ATPase [J].

Abrahams, JP ;

Leslie, AGW .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 :30-42

[2] THE CHYMOTRYPSIN-LIKE ACTIVITY OF HUMAN PROSTATE-SPECIFIC ANTIGEN, GAMMA-SEMINOPROTEIN [J].

AKIYAMA, K ;

NAKAMURA, T ;

IWANAGA, S ;

HARA, M .

FEBS LETTERS, 1987, 225 (1-2) :168-172

[3]

[Anonymous], [No title captured]

[4]

BHOOLA KD, 1992, PHARMACOL REV, V44, P1

[5] REFINED 2 A X-RAY CRYSTAL-STRUCTURE OF PORCINE PANCREATIC KALLIKREIN-A, A SPECIFIC TRYPSIN-LIKE SERINE PROTEINASE - CRYSTALLIZATION, STRUCTURE DETERMINATION, CRYSTALLOGRAPHIC REFINEMENT, STRUCTURE AND ITS COMPARISON WITH BOVINE TRYPSIN [J].

BODE, W ;

CHEN, ZG ;

BARTELS, K ;

KUTZBACH, C ;

SCHMIDTKASTNER, G ;

BARTUNIK, H .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 164 (02) :237-282

[6] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[7] ISOLATION AND BIOCHEMICAL-CHARACTERIZATION OF STALLION SEMINAL-PLASMA PROTEINS [J].

CALVETE, JJ ;

NESSAU, S ;

MANN, K ;

SANZ, L ;

SIEME, H ;

KLUG, E ;

TOPFERPETERSEN, E .

REPRODUCTION IN DOMESTIC ANIMALS, 1994, 29 (06) :411-426

[8] REFINED 2.5 A X-RAY CRYSTAL-STRUCTURE OF THE COMPLEX FORMED BY PORCINE KALLIKREIN-A AND THE BOVINE PANCREATIC TRYPSIN-INHIBITOR - CRYSTALLIZATION, PATTERSON SEARCH, STRUCTURE DETERMINATION, REFINEMENT, STRUCTURE AND COMPARISON WITH ITS COMPONENTS AND WITH THE BOVINE TRYPSIN PANCREATIC TRYPSIN-INHIBITOR COMPLEX [J].

CHEN, ZG ;

BODE, W .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 164 (02) :283-311

[9] ENZYMATIC-ACTIVITY OF PROSTATE-SPECIFIC ANTIGEN AND ITS REACTIONS WITH EXTRACELLULAR SERINE PROTEINASE-INHIBITORS [J].

CHRISTENSSON, A ;

LAURELL, CB ;

LILJA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 194 (03) :755-763

[10] THE HUMAN KALLIKREIN GENE FAMILY - A DIVERSITY OF EXPRESSION AND FUNCTION [J].

CLEMENTS, JA .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1994, 99 (01) :C1-C6

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