Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

被引：2

作者：

Carvalho, AL

Dias, JM

Sanz, L

Romero, A

Calvete, JJ

Romao, MJ ^{[1
]}

机构：

[1] Univ Nova Lisboa, Fac Ciencias & Tecnol, Ctr Quim Fina & Biotecnol, Dept Quim, P-2829516 Monte De Caparica, Portugal

[2] CSIC, Inst Biomed Valencia, E-46010 Valencia, Spain

[3] CSIC, Ctr Invest Biol, E-28006 Madrid, Spain

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901009805

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purired from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Angstrom resolution were collected at the ESRF synchrotron-radiation source.

引用

页码：1180 / 1183

页数：4

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