Characterization of glutamate dehydrogenase immobilization on silica surface by atomic force microscopy and kinetic analyses

被引：15

作者：

Blasi, L ^{[1
]}

Longo, L

Vasapollo, G

Cingolani, R

Rinaldi, R

Rizzello, T

Acierno, R

Maffia, M

机构：

[1] CO Dept Innovat Engn, Univ Lecce, INFM, Natl Nanotechnol Lab, Lecce, Italy

[2] Univ Lecce, Lab Gen Physiol, Dept Biol & Environm Sci & Technol, I-73100 Lecce, Italy

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2005年 / 36卷 / 5-6期

关键词：

glutamate dehydrogenase; atomic force microscopy; enzymatic assays;

D O I：

10.1016/j.enzmictec.2005.01.014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Covalent immobilization of glutamate dehydrogenase (GDH) onto activated Si/SiO2 supports was analyzed by both atomic force microscopy (AFM) and an enzymatic assay. When the concentration of 3-aminopropyltriethoxysilane used in the first derivatization step of the silicon surface was decreased, the specific enzymatic activity also decreased, whereas the mean roughness increased. Thus, the activity of immobilized GDH is critically dependent on the conditions for surface derivatization, and is inversely correlated with surface roughness. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：818 / 823

页数：6

共 21 条

[1]

[Anonymous], IMMOBILIZATION ENZYM, DOI [10.1385/0-89603-386-4:21, DOI 10.1385/0-89603-386-4:21]

[2]

COLOWICK SP, 1985, METHOD ENZYMOL, V113, P16

[3]

Direct immobilization of glutamate dehydrogenase on optical fiber probes for ultrasensitive glutamate detection [J].

Cordek, J ;

Wang, XW ;

Tan, WH .

ANALYTICAL CHEMISTRY, 1999, 71 (08) :1529-1533

[4]

FISHER HF, 1985, METHOD ENZYMOL, V113, P16

[5]

Gooding JJ, 1998, ELECTROANAL, V10, P1130, DOI 10.1002/(SICI)1521-4109(199811)10:16<1130::AID-ELAN1130>3.3.CO

[6]

2-Y

[7]

Disorders of glutamate metabolism [J].

Kelly, A ;

Stanley, CA .

MENTAL RETARDATION AND DEVELOPMENTAL DISABILITIES RESEARCH REVIEWS, 2001, 7 (04) :287-295

[8]

Conformation, orientation, and adsorption kinetics of dermaseptin B2 onto synthetic supports at aqueous/solid interface [J].

Noinville, S ;

Bruston, F ;

El Amri, C ;

Baron, D ;

Nicolas, P .

BIOPHYSICAL JOURNAL, 2003, 85 (02) :1196-1206

[9]

The structure of bovine glutamate dehydrogenase provides insights into the mechanism of allostery [J].

Peterson, PE ;

Smith, TJ .

STRUCTURE, 1999, 7 (07) :769-782

[10]

Crystallization and characterization of bovine liver glutamate dehydrogenase [J].

Peterson, PE ;

Pierce, J ;

Smith, TJ .

JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (01) :73-77

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