Crystallization and characterization of bovine liver glutamate dehydrogenase

被引：29

作者：

Peterson, PE ^{[1
]}

Pierce, J ^{[1
]}

Smith, TJ ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 120卷 / 01期

关键词：

D O I：

10.1006/jsbi.1997.3899

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Bovine liver glutamate dehydrogenase has been crystallized as an abortive complex with glutamic acid, NADH, and an inhibitor, GTP. Crystals of this complex were grown using the sitting drop vapor diffusion method with PEG 8000 as the precipitant and diffract to better than 2.5 Angstrom resolution. The crystals belong to the space group P2(1) with an entire enzyme hexamer in the crystallographic asymmetric unit. Self-rotation and self-Patterson functions clearly define the orientation and position of this hexameric enzyme. (C) 1997 Academic Press.

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页码：73 / 77

页数：5

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