Sulphatide and its precursor galactosylceramide influence the production of cytokines in human mononuclear cells

被引:23
作者
Buschard, K
Diamant, M
Bovin, LF
Mansson, JE
Fredman, P
Bendtzen, K
机构
[1] NATL UNIV HOSP COPENHAGEN,INST INFLAMMAT RES,COPENHAGEN,DENMARK
[2] UNIV GOTHENBURG,DEPT CLIN NEUROSCI,SECT PSYCHIAT & NEUROCHEM,MOLNDAL,SWEDEN
关键词
sulphatide; galactosylceramide; blood mononuclear cells; cytokines; interleukin-6 (IL-6); IL-6; mRNA;
D O I
10.1111/j.1699-0463.1996.tb04962.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Sulphatide is expressed in the central and peripheral neural system, in islets of Langerhans, and in tissues affected by late diabetic complications. Autoantibodies to sulphatide are present in patients with insulin-dependent diabetes and the Guillain-Ban-C syndrome. Cytokines influence these disease processes, and we therefore studied whether sulphatide and its precursor galactosylceramide (gal-cer) influence the in vitro production of cytokines by blood monunuclear cells (MNC) originating from 15 healthy persons. Using lipopolysaccharide (LPS)-stimulated cells, sulphatide increased the IL-2. production (163 +/- 17% of controls without sulphatide. p = 0.02), and gal-cer increased the IL-1 alpha production (145 +/- 13%, p = 0.006), whereas neither gal-cer nor sulphatide had an effect on the production of IL-6, IL-10 or TNF alpha. When stimulating cells with phytohaemagglutinin (PHA), sulphatide decreased the production of IL-6 (88 +/- 5%, p = 0.009), IL-10 (66 +/- 3%, p = 0.000003), and TNF alpha (75 +/- 9%: p = 0.02). Gal-cer, however. increased the production of IL-6 (188 +/- 13%, p = 0.000006), and decreased the production of TNF beta (80 +/- 6%, p = 0.007). Neither gal-cer nor sulphatide had an effect on the production of IL-2 or IFN gamma from PHA-stimulated cells. Northern blot analysis using an IL-6 probe similarly showed an increased amount of IL-6 mRNA after gal-cer incubation (range 469%-150%, n = 3) of PHA-stimulated control. Thus, sulphatide and gal-cer influence the production of several cytokines thought to be involved in immunoinflammatory disease processes.
引用
收藏
页码:938 / 944
页数:7
相关论文
共 28 条
[11]  
HEEGAARD PMH, 1988, EOS J IMMUNOL IMMUNO, V4, P239
[12]  
LAUDANNA C, 1994, J BIOL CHEM, V269, P4021
[13]   CERAMIDE INDUCES INTERLEUKIN-6 GENE-EXPRESSION IN HUMAN FIBROBLASTS [J].
LAULEDERKIND, SJF ;
BIELAWSKA, A ;
RAGHOW, R ;
HANNUM, YA ;
BALLOU, LR .
JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 182 (02) :599-604
[14]   Cytokines and the endocrine system .2. Roles in substrate metabolism, modulation of thyroidal and pancreatic endocrine cell functions and autoimmune endocrine diseases [J].
MandrupPoulsen, T ;
Nerup, J ;
Reimers, JI ;
Pociot, F ;
Andersen, HU ;
Karlsen, A ;
Bjerre, U ;
Bergholdt, R .
EUROPEAN JOURNAL OF ENDOCRINOLOGY, 1996, 134 (01) :21-30
[15]   TRISIALOSYLLACTOSYLCERAMIDE (GT3) IS A GANGLIOSIDE OF HUMAN-LUNG [J].
MANSSON, JE ;
MO, H ;
EGGE, H ;
SVENNERHOLM, L .
FEBS LETTERS, 1986, 196 (02) :259-262
[16]   ACTIVATION OF THE SPHINGOMYELIN SIGNALING PATHWAY IN INTACT EL4 CELLS AND IN A CELL-FREE SYSTEM BY IL-1-BETA [J].
MATHIAS, S ;
YOUNES, A ;
KAN, CC ;
ORLOW, I ;
JOSEPH, C ;
KOLESNICK, RN .
SCIENCE, 1993, 259 (5094) :519-522
[17]   ANALYSIS OF SINGLE-STRANDED AND DOUBLE-STRANDED NUCLEIC-ACIDS ON POLYACRYLAMIDE AND AGAROSE GELS BY USING GLYOXAL AND ACRIDINE-ORANGE [J].
MCMASTER, GK ;
CARMICHAEL, GG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :4835-4838
[18]   IN-VITRO IMMUNOMODULATORY EFFECTS OF PENTOXIFYLLINE [J].
RIENECK, K ;
DIAMANT, M ;
HAAHR, PM ;
SCHONHARTING, M ;
BENDTZEN, K .
IMMUNOLOGY LETTERS, 1993, 37 (2-3) :131-138
[19]   Cytokines and their receptors in the central nervous system: Physiology, pharmacology, and pathology [J].
Rothwell, NJ ;
Luheshi, G ;
Toulmond, S .
PHARMACOLOGY & THERAPEUTICS, 1996, 69 (02) :85-95
[20]   INTERLEUKIN-6 AFFECTS INSULIN-SECRETION AND GLUCOSE-METABOLISM OF RAT PANCREATIC-ISLETS INVITRO [J].
SANDLER, S ;
BENDTZEN, K ;
EIZIRIK, DL ;
WELSH, M .
ENDOCRINOLOGY, 1990, 126 (02) :1288-1294