The Sulfolobus solfataricus P2 genome project

被引：17

作者：

Charlebois, RL

Gaasterland, T

Ragan, MA

Doolittle, WF

Sensen, CW

机构：

[1] UNIV OTTAWA,DEPT BIOL,OTTAWA,ON K1N 6N5,CANADA

[2] ARGONNE NATL LAB,DIV MATH & COMP SCI,ARGONNE,IL 60439

[3] UNIV CHICAGO,DEPT COMP SCI,CHICAGO,IL 60637

[4] NATL RES COUNCIL CANADA,INST MARINE BIOSCI,HALIFAX,NS B3H 3Z1,CANADA

[5] DALHOUSIE UNIV,DEPT BIOCHEM,HALIFAX,NS B3H 4H7,CANADA

来源：

FEBS LETTERS | 1996年 / 389卷 / 01期

关键词：

Archaea; genome sequencing; Sulfolobus solfataricus; thermophilic Crenarchaeota;

D O I：

10.1016/0014-5793(96)00525-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over 800 kbp of the 3-Mbp genome of Sulfolobus solfataricus have been sequenced to date, Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers, Using a prototype automated system for genome-scale analysis, known as MAGPIE, along with other toots, we have discovered one open reading frame of at least 100 amino acids per kbp of sequence, and have been able to associate 50% of these with known genes through database searches, An examination of completely sequenced cosmids suggests a clustering of genes by function in the S, solfataricus genome.

引用

页码：88 / 91

页数：4

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