Polypyrimidine tract-binding protein inhibits translation of Bip mRNA

被引：62

作者：

Kim, YK ^{[1
]}

Hahm, B ^{[1
]}

Jang, SK ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Dept Life Sci, NRL, Pohang 790784, Kyungbuk, South Korea

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2000年 / 304卷 / 02期

关键词：

translation; Bip; IRES; PTB;

D O I：

10.1006/jmbi.2000.4179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding protein (PTB) has been found to enhance the translation through IRES elements, probably by interaction with the IRES structure. Here, we report that PTB specifically binds to the central region (nt 50-117) of the Bip 5' non-translated region. Addition of purified PTB to rabbit reticulocyte lysate and overexpression of PTB in Cos-7 cells selectively inhibited Bip IRES-dependent translation On the other hand, depletion of endogenous PTB or addition of an RNA interacting with PTB enhanced the translational initiation directed by Bip IRES. These suggest that PTB can either enhance or inhibit IRES-dependent translation depending on mRNAs. (C) 2000 Academic Press.

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页码：119 / 133

页数：15

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