Ceftazidime-resistant Klebsiella pneumoniae and Escherichia coli isolates producing TEM-10 and TEM-43 β-lactamases from St. Louis, Missouri

被引:41
作者
Yang, YJ
Bhachech, N
Bradford, PA
Jett, BD
Sahm, DF
Bush, K
机构
[1] Wyeth Ayerst Res, Infect Dis Res, Pearl River, NY 10965 USA
[2] Washington Univ, Barnes Jewish Hosp, Sch Med, St Louis, MO USA
关键词
D O I
10.1128/AAC.42.7.1671
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae (49 and 102 isolates, respectively) were collected from Barnes-Jewish Hospital, St. Louis, Mo., from 1992 to 1996, They were uniformly resistant to ceftazidime, generally resistant to aztreonam, and variably susceptible to cefotaxime, Four representative E, coli strains and 15 Klebsiella strains were examined, From one to four beta-lactamases were produced per strain, with three possible enzymes related to ceftazidime resistance: enzymes with pi values of 5.6, 6.1, or 7.6. By pulsed-field gel electrophoresis there were at least 13 different Klebsiella strain types and 3 different E, coli strain types, indicating that the outbreak was not clonal, After cloning and sequencing of the beta-lactamase-encoding genes, the enzyme with a pi of 5.6 was identified as TEM-10, The enzyme with a pi of 6.1 was a novel TEM variant (TEM-43) with Lys at 104, His at 164, and Thr at 182, TEM-43 showed broad-spectrum hydrolytic activity against all penicillins, with the highest hydrolysis rate for ceftazidime compared to those for the other expanded-spectrum cephalosporins. Aztreonam was also a good substrate for TEM-43, with hydrolytic activity similar to that of ceftazidime and affinity higher than that of ceftazidime. The TEM-43 beta-lactamase was well inhibited by clavulanic acid and tazobactam at concentrations of <10 nM, Sulbactam was less effective than the other inhibitors. The Thr182 mutation previously reported in an inhibitor-resistant beta-lactamase did not cause the TEM-43 enzyme to become resistant to any of the inhibitors.
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页码:1671 / 1676
页数:6
相关论文
共 32 条
[1]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[2]   CHARACTERIZATION OF A NEW TEM-TYPE BETA-LACTAMASE RESISTANT TO CLAVULANATE, SULBACTAM, AND TAZOBACTAM IN A CLINICAL ISOLATE OF ESCHERICHIA-COLI [J].
BLAZQUEZ, J ;
BAQUERO, MR ;
CANTON, R ;
ALOS, I ;
BAQUERO, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (10) :2059-2063
[3]   MULTIPLY RESISTANT KLEBSIELLA-PNEUMONIAE STRAINS FROM 2 CHICAGO HOSPITALS - IDENTIFICATION OF THE EXTENDED-SPECTRUM TEM-12 AND TEM-10 CEFTAZIDIME-HYDROLYZING BETA-LACTAMASES IN A SINGLE ISOLATE [J].
BRADFORD, PA ;
CHERUBIN, CE ;
IDEMYOR, V ;
RASMUSSEN, BA ;
BUSH, K .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (04) :761-766
[4]   TEM-28 from an Escherichia coli clinical isolate is a member of the his-164 family of TEM-1 extended-spectrum beta-lactamases [J].
Bradford, PA ;
Jacobus, NV ;
Bhachech, N ;
Bush, K .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (01) :260-262
[5]   SHV-7, A NOVEL CEFOTAXIME-HYDROLYZING BETA-LACTAMASE, IDENTIFIED IN ESCHERICHIA-COLI ISOLATES FROM HOSPITALIZED NURSING-HOME PATIENTS [J].
BRADFORD, PA ;
URBAN, C ;
JAISWAL, A ;
MARIANO, N ;
RASMUSSEN, BA ;
PROJAN, SJ ;
RAHAL, JJ ;
BUSH, K .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (04) :899-905
[6]   A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].
BUSH, K ;
JACOBY, GA ;
MEDEIROS, AA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233
[7]   Implication of Ile-69 and Thr-182 residues in kinetic characteristics of IRT-3 (TEM-32) beta-lactamase [J].
Farzaneh, S ;
Chaibi, EB ;
Peduzzi, J ;
Barthelemy, M ;
Labia, R ;
Blazquez, J ;
Baquero, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (10) :2434-2436
[8]   MOLECULAR CHARACTERIZATION OF 9 DIFFERENT TYPES OF MUTANTS AMONG 107 INHIBITOR-RESISTANT TEM BETA-LACTAMASES FROM CLINICAL ISOLATES OF ESCHERICHIA-COLI [J].
HENQUELL, C ;
CHANAL, C ;
SIROT, D ;
LABIA, R ;
SIROT, J .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (02) :427-430
[9]   A natural polymorphism in beta-lactamase is a global suppressor [J].
Huang, WZ ;
Palzkill, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (16) :8801-8806
[10]   MORE EXTENDED-SPECTRUM BETA-LACTAMASES [J].
JACOBY, GA ;
MEDEIROS, AA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (09) :1697-1704