Two-Photon Excited Fluorescence of a Conjugated Polyelectrolyte and Its Application in Cell Imaging

被引：43

作者：

Parthasarathy, Anand

Ahn, Hyo-Yang

Belfield, Kevin D.

Schanze, Kirk S. ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Ctr Macromol Sci & Engn, Gainesville, FL 32611 USA

来源：

ACS APPLIED MATERIALS & INTERFACES | 2010年 / 2卷 / 10期

基金：

美国国家科学基金会;

关键词：

two-photon absorption; conjugated polyelectrolytes; near-infrared; cell imaging; OPTICAL NONLINEARITY; ABSORPTION; POLYMERS; SPECTROSCOPY; PHOTOPHYSICS; SHELL; STATE;

D O I：

10.1021/am100784m

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in He La cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

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收藏

页码：2744 / 2748

页数：5

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