Effect of bile salts on colonic mucus secretion in isolated vascularly perfused rat colon

被引:26
作者
Barcelo, A
Claustre, J
Toumi, F
Burlet, G
Chayvialle, JA
Cuber, JC
Plaisancié, P
机构
[1] Hop Edouard Herriot, INSERM U45, F-69437 Lyon 03, France
[2] INRA, Lab Ecol & Physiol Syst Digest, Ctr Rech, Jouy En Josas, France
关键词
colon; mucus secretion; goblet cell; rat; bile salts;
D O I
10.1023/A:1010607127822
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Mainly composed of mucins, mucus secreted by goblet cells in the intestinal epithelium is critically involved in the protection of the gastrointestinal mucosa. The hypothesis that bile and some bile salts can induce mucus secretion was tested in the isolated perfused rat colon. Mucus release was evaluated using enzyme-linked immunosorbent assays and supported by histological analysis. Luminal administration of bile extract (1%) provoked mucus secretion in the rat colon. Deoxycholate (0.5-10 mM) induced a dose-dependent increase in rat colonic mucus release. Chenodeoxycholate (10 mM) and hyodeoxycholate (10 mM) also evoked mucus discharge, whereas 10 mM cholate, 10 mM ursodeoxycholate, or Tween-20 did not release mucus. Taurine-conjugated bile salts (deoxycholate, hyodeoxycholate, and chenodeoxycholate) were less potent mucus secretagogues than the corresponding unconjugated forms. The deoxycholate-induced mucus discharge was not altered by pharmacological blockers (tetrodotoxin, atropine), indomethacin, mast cell stabilizers (ketotifen, doxantrazole), H, histamine receptor antagonist (pyrilamine), or 5-HT receptor antagonists (ketanserin, ondansetron, SDZ 205-557), Our findings suggest that some bile salts, especially in the unconjugated form, may provoke colonic mucus secretion, probably through a direct action on mucus-secreting cells.
引用
收藏
页码:1223 / 1231
页数:9
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