Anticoagulant dermatan sulfate proteoglycan (decorin) in the term human placenta

被引:36
作者
Delorme, MA
Xu, L
Berry, L
Mitchell, L
Andrew, M
机构
[1] Univ Western Ontario, St Josephs Hlth Ctr, Div Hematol Oncol, Dept Med, London, ON N6A 4V2, Canada
[2] McMaster Univ, Dept Pediat, Hamilton, ON, Canada
基金
英国医学研究理事会;
关键词
dermatan sulfate; placenta; proteoglycan; heparin cofactor II;
D O I
10.1016/S0049-3848(98)00035-8
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Normal pregnancy is characterized by increased in vivo thrombin generation. A greater proportion of endogenously generated thrombin is complexed to heparin cofactor II in plasma from pregnant women compared to plasma from nonpregnant ones, The increase in thrombin-heparin cofactor II complexes suggests that the site of the additional thrombin generation is relatively rich in dermatan sulfate. We postulated that the site of thrombin generation may be the placenta since endogenous thrombin generation returns rapidly to normal after delivery. This report describes the isolation and characterization of placental dermatan sulfate proteoglycan from villous tissue of the term human placenta, Placental dermatan sulfate was isolated bq. guanidine HCl extraction and anion exchange chromatography, The isolated material was found to have anticoagulant activity with a relative activity of approximately 40% of that of a porcine mucosal dermatan sulfate which is undergoing clinical trial as an antithrombotic agent. The dermatan sulfate was present as a proteoglycan with a molecular mass of 90-150 kD, Upon degradation with chondroitin ABC lyase, the core protein was demonstrated to be a doublet with molecular masses of 42 and 44 kD, This core protein reacted with antiserum against the core protein of decorin on Western analysis. The role of this placental dermatan sulfate in local regulation of thrombin in the placenta warrants further study. (C) 1998 Elsevier Science Ltd.
引用
收藏
页码:147 / 153
页数:7
相关论文
共 21 条
[1]   AN ANTICOAGULANT DERMATAN SULFATE PROTEOGLYCAN CIRCULATES IN THE PREGNANT WOMAN AND HER FETUS [J].
ANDREW, M ;
MITCHELL, L ;
BERRY, L ;
PAES, B ;
DELORME, M ;
OFOSU, F ;
BURROWS, R ;
KHAMBALIA, B .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (01) :321-326
[2]   A MICRODETERMINATION METHOD FOR ASSAYING GLYCOSAMINOGLYCANS AND PROTEOGLYCANS [J].
BARTOLD, PM ;
PAGE, RC .
ANALYTICAL BIOCHEMISTRY, 1985, 150 (02) :320-324
[3]  
BRENNAN MJ, 1984, J BIOL CHEM, V259, P3742
[4]  
Delorme MA, 1996, THROMB HAEMOSTASIS, V75, P747
[5]  
DUPOUY D, 1988, THROMB HAEMOSTASIS, V60, P236
[6]  
FAULK WP, 1990, BLOOD, V76, P86
[7]  
GERBASI FR, 1990, OBSTET GYNECOL, V75, P385
[8]  
GRIFFITH MJ, 1985, J BIOL CHEM, V260, P2218
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   CHANGES IN COMPOSITION AND STRUCTURE OF GLYCOSAMINOGLYCANS IN HUMAN PLACENTA DURING DEVELOPMENT [J].
LEE, TY ;
JAMIESON, AM ;
SCHAFER, IA .
PEDIATRIC RESEARCH, 1973, 7 (12) :965-977