Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae

被引：73

作者：

Laadan, Boaz ^{[1
]}

Almeida, Joao R. M. ^{[1
]}

Radstrom, Peter ^{[1
]}

Hahn-Hagerdal, Barbel ^{[1
]}

Gorwa-Grauslund, Marie ^{[1
]}

机构：

[1] Lund Univ, Dept Appl Microbiol, SE-22100 Lund, Sweden

来源：

YEAST | 2008年 / 25卷 / 03期

关键词：

alcohol dehydrogenase; 5-hydroxymethylfurfural; detoxification; lignocellulosic hydrolysates;

D O I：

10.1002/yea.1578

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the identification and characterization of a mutated alcohol dehydrogenase I from the industrial Saccharomyces cerevisiae strain TMB3000 that mediates the NADII-dependent reduction of 5-hydroxymethyllfurfural (HMF) to 2,5-bis-hydroxymethylfuran. The co-factor preference distinguished this alcohol dehydrogenase from the previously reported NADPH-dependent S. cerevisiae HMF alcohol dehydrogenase Adh6. The amino acid sequence revealed three novel mutations (S109P, L116S and Y294C) that were all predicted at the vicinity of the substrate binding site, which could explain the unusual substrate specificity. Increased biomass production and HMF conversion rate were achieved in a CEN.PK S. cerevisiae strain overexpressing the mutated ADHI gene. Copyright (c) 2008 John Wiley & Sons, Ltd.

引用

页码：191 / 198

页数：8

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