Characterization of tau in cerebrospinal fluid using mass spectrometry

被引：63

作者：

Portelius, Erik ^{[1
]}

Hansson, Sara F. ^{[1
]}

Tran, Ai Jun ^{[1
]}

Zetterberg, Henrik ^{[1
]}

Grognet, Pierre ^{[2
]}

Vanmechelen, Eugeen ^{[2
]}

Hoglund, Kina ^{[3
]}

Brinkmahn, Gunnar ^{[1
]}

Westman-Brinkmalm, Ann ^{[1
]}

Nordhoff, Eckhard ^{[4
]}

Blennow, Kai ^{[1
]}

Gobom, Johan ^{[1
]}

机构：

[1] Univ Gothenburg, Sahlgrens Univ Hosp, Dept Neurosci & Physiol, Clin Neurochem Lab, S-43180 Molndal, Sweden

[2] Innogenet, Ghent, Belgium

[3] AstraZeneca CNS Pain, Dept Dis Biol, SE-15185 Sodertalje, Sweden

[4] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 05期

关键词：

Alzheimer's disease; immunoprecipitation; MALDI-TOF MS; cerebrospinal fluid; tau; LC-MS/MS;

D O I：

10.1021/pr7008669

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The neurodegenerative disorder Alzheimer's disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.

引用

页码：2114 / 2120

页数：7

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