Prevalence and genetic diversity of adeno-associated viruses in bats from China

被引：28

作者：

Li, Yan ^{[1
]}

Ge, Xingyi ^{[1
]}

Hon, Chung-Chau ^{[2
]}

Zhang, Huajun ^{[1
]}

Zhou, Peng ^{[1
]}

Zhang, Yunzhi ^{[1
,3
]}

Wu, Yi ^{[4
]}

Wang, Lin-Fa ^{[4
]}

Shi, Zhengli ^{[1
]}

机构：

[1] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China

[2] INSERM, U389, Unite Biol Cellulaire Parasitisme, F-75654 Paris 13, France

[3] Yunnan Inst Endem Dis Control & Prevent, Dali, Peoples R China

[4] Australian Anim Hlth Lab, CSIRO Livestock Ind, Geelong, Vic, Australia

来源：

JOURNAL OF GENERAL VIROLOGY | 2010年 / 91卷

基金：

中国国家自然科学基金;

关键词：

DNA AMPLIFICATION; HUMAN TISSUES; PARVOVIRUS; CLONING; PARTICLES; ADENOVIRUS; SEQUENCES; THERAPY; CELLS; RECOMBINATION;

D O I：

10.1099/vir.0.020032-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bats are increasingly being recognized as important natural reservoirs of different viruses Adeno-associated viruses (AAVs) are widely distributed in primates and their distribution in bats is unknown. In this study, a total of 370 faecal swab samples from 19 bat species were collected from various provinces of China and examined for the presence of AAVs The mean prevalence rate was 22.4 % (83 positives out of 370 samples), ranging from 10 to 38 9% among different bat species The genome sequence spanning the entire rep-cap ORFs was determined from one chosen AAV-positive sample (designated BtAAV-YNM). Phylogenetic analysis of the entire rep-cap ORF coding sequences suggested that BtAAV-YNM is relatively distant to known primate AAVs, but phylogenetically closer to porcine AAV strain Po3 Further analysis of the partial cap ORF sequences of bat AAV samples (n=49) revealed a remarkably large genetic diversity, with an average pairwise nucleotide identity of only 84 3% Co-presence of multiple distinctive genotypes of bat AAV within an individual sample was also observed. These results demonstrated that diverse AAVs might be widely distributed in bat populations

引用

页码：2601 / 2609

页数：9