Stage-specific translational efficiency and protein stability regulate the developmental expression of p37, an RNA binding protein from Trypanosoma brucei

被引:5
作者
Li, JL
Ruyechan, WT
Williams, N
机构
[1] SUNY Buffalo, Dept Microbiol, Buffalo, NY 14214 USA
[2] SUNY Buffalo, Witebsky Ctr Microbial Pathogenesis & Immunol, Buffalo, NY 14214 USA
关键词
Trypanosoma brucei; RNA binding protein; developmental regulation; translational regulation; post-translational regulation;
D O I
10.1016/S0006-291X(03)01084-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously characterized two novel RNA binding proteins, p34 and p37, from Trypanosoma brucei. Their sequences do not show significant homology to other proteins but are highly homologous to one another. The p34 and p37 proteins are developmentally regulated, with p34 the predominant protein in the procyclic stage and p37 nearly exclusively expressed in the bloodstream cells. In vivo metabolic labeling of procyclic cells showed that p34 and p37 were differentially translated, with levels of p34 approximately fourfold higher than p37. The newly synthesized p34 and p37 exhibited differential stability in the procyclic stage. In vitro analysis confirmed this observation and further suggested that this differential stability may be due to a trypsin-like cysteine protease activity in procyclic extracts that selectively degraded the p37 protein. Taken together, these results indicate that the developmental regulation of the T brucei RNA binding protein, p37, occurs at both translational and post-translational levels. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:918 / 923
页数:6
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