Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes

被引：8

作者：

Huang, CY ^{[1
]}

Kasai, M ^{[1
]}

Buetow, DE ^{[1
]}

机构：

[1] Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1998年 / 14卷 / 04期

关键词：

dot blot; mammalian tissue RNAs; non-radioactive probes; rapid detection;

D O I：

10.1016/S1050-3862(98)00003-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 a for even low abundance mRNA. (C) 1998 Elsevier Science B.V. Al rights reserved.

引用

页码：109 / 112

页数：4