Super-resolution track-density imaging studies of mouse brain: Comparison to histology

被引:88
作者
Calamante, Fernando [1 ,2 ]
Tournier, Jacques-Donald [1 ,2 ]
Kurniawan, Nyoman D. [3 ]
Yang, Zhengyi [3 ]
Gyengesi, Erika [4 ]
Galloway, Graham J. [3 ]
Reutens, David C. [3 ]
Connelly, Alan [1 ,2 ]
机构
[1] Melbourne Brain Ctr, Florey Neurosci Inst, Brain Res Inst, Heidelberg, Vic 3084, Australia
[2] Univ Melbourne, Dept Med, Melbourne, Vic, Australia
[3] Univ Queensland, Ctr Adv Imaging, Brisbane, Qld, Australia
[4] Neurosci Res Australia, Randwick, NSW, Australia
基金
英国医学研究理事会;
关键词
Magnetic resonance imaging; Super-resolution; Mouse brain; Diffusion MRI; Validation; Histology; SPHERICAL DECONVOLUTION; DIFFUSION; ORIENTATION; MRI;
D O I
10.1016/j.neuroimage.2011.07.014
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The recently proposed track-density imaging (TDI) technique was introduced as a means to achieve super-resolution using diffusion MRI. This technique is able to increase the spatial resolution of the reconstructed images beyond the acquired MRI resolution by incorporating information from whole-brain fibre-tracking results. It not only achieves super-resolution, but also provides very high anatomical contrast with a new MRI contrast mechanism. However, the anatomical information-content of this novel contrast mechanism has not yet been assessed. In this work, we perform such a study using diffusion MRI of ex vivo mouse brains acquired at 16.4T, to compare the results of the super-resolution TDI technique with histological staining (myelin and Nissl stains) in the same brains. Furthermore, a modified version of the directionally-encoded colour TDI map using short-tracks is introduced, which reduces the TDI intensity dynamic range, and therefore enhances the directionality colour-contrast. Good agreement was observed between structures visualised in the super-resolution TDI maps and in the histological sections, supporting the anatomical information-content of the images generated using the TDI technique. The results therefore show that the TDI methodology does provide meaningful and rich anatomical contrast, in addition to achieving super-resolution. Furthermore, this study is the first to show the application of TDI to mouse brain imaging: the high-resolution, high-quality images demonstrate the useful complementary information that can be achieved using super-resolution TDI. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:286 / 296
页数:11
相关论文
共 21 条
[1]   Three-Dimensional Diffusion Tensor Microimaging for Anatomical Characterization of the Mouse Brain [J].
Aggarwal, Manisha ;
Mori, Susumu ;
Shimogori, Tomomi ;
Blackshaw, Seth ;
Zhang, Jiangyang .
MAGNETIC RESONANCE IN MEDICINE, 2010, 64 (01) :249-261
[2]   Non-invasive mapping of connections between human thalamus and cortex using diffusion imaging [J].
Behrens, TEJ ;
Johansen-Berg, H ;
Woolrich, MW ;
Smith, SM ;
Wheeler-Kingshott, CAM ;
Boulby, PA ;
Barker, GJ ;
Sillery, EL ;
Sheehan, K ;
Ciccarelli, O ;
Thompson, AJ ;
Brady, JM ;
Matthews, PM .
NATURE NEUROSCIENCE, 2003, 6 (07) :750-757
[3]   Track density imaging (TDI): Validation of super resolution property [J].
Calamante, Fernando ;
Tournier, Jacques-Donald ;
Heidemann, Robin M. ;
Anwander, Alfred ;
Jackson, Graeme D. ;
Connelly, Alan .
NEUROIMAGE, 2011, 56 (03) :1259-1266
[4]   Track-density imaging (TDI): Super-resolution white matter imaging using whole-brain track-density mapping [J].
Calamante, Fernando ;
Tournier, Jacques-Donald ;
Jackson, Graeme D. ;
Connelly, Alan .
NEUROIMAGE, 2010, 53 (04) :1233-1243
[5]   An MRI-based atlas and database of the developing mouse brain [J].
Chuang, Nelson ;
Mori, Susumu ;
Yamamoto, Akira ;
Jiang, Hangyi ;
Ye, Xin ;
Xu, Xin ;
Richards, Linda J. ;
Nathans, Jeremy ;
Miller, Michael I. ;
Toga, Arthur W. ;
Sidman, Richard L. ;
Zhang, Jiangyang .
NEUROIMAGE, 2011, 54 (01) :80-89
[6]   A software tool to generate simulated white matter structures for the assessment of fibre-tracking algorithms [J].
Close, Thomas G. ;
Tournier, Jacques-Donald ;
Calamante, Fernando ;
Johnston, Leigh A. ;
Mareels, Iven ;
Connelly, Alan .
NEUROIMAGE, 2009, 47 (04) :1288-1300
[7]  
GALLYAS F, 1971, ACTA MORPHOL HUNG, V19, P57
[8]  
Gallyas F, 1979, Neurol Res, V1, P203
[9]   Microscopic diffusion tensor imaging of the mouse brain [J].
Jiang, Yi ;
Johnson, G. Allan .
NEUROIMAGE, 2010, 50 (02) :465-471
[10]  
Jones DK, 1999, MAGNET RESON MED, V42, P515, DOI 10.1002/(SICI)1522-2594(199909)42:3<515::AID-MRM14>3.0.CO