共 43 条
Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function
被引:48
作者:

Campbell, TL
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

Daigle, DM
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

Brown, ED
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada
机构:
[1] McMaster Univ, Antimicrobial Res Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada
关键词:
antibiotic;
gene deletion;
GTPase;
polyribosome profile;
ribosome;
YloQ;
D O I:
10.1042/BJ20041873
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We present an analysis of the cellular phenotype and biochemical activity of a conserved bacterial GTPase of unknown function (YloQ and YjeQ in Bacillus subtilis and Escherichia coli respectively) using a collection of antibiotics of diverse mechanisms and chemical classes. We created a yloQ deletion strain, which exhibited a slow growth phenotype and formed chains of filamentous cells. Additionally, we constructed a conditional mutant in yloQ, where growth was dependent on inducible expression from a complementing copy of the gene. In phenotypic studies, depletion of yloQ sensitized cells to antibiotics that bind at the peptide channel or peptidyl transferase centre, providing the first chemical genetic evidence linking this GTPase to ribosome function. Additional experiments using these small-molecule probes in vitro revealed that aminoglycoside antibiotics severely affected a previously characterized ribosome-associated GTPase activity of purified, recombinant YjeQ from E. coli. None of the antibiotics tested competed with YjeQ for binding to 30 or 70 S ribosomes. A closer examination of YloQ depletion revealed that the polyribosome profiles were altered and that decreased expression of YloQ led to the accumulation of ribosomal subunits at the expense of intact 70 S ribosomes. The present study provides the first evidence showing that YloQ/YjeQ may be involved in several areas of cellular metabolism, including cell division and ribosome function.
引用
收藏
页码:843 / 852
页数:10
相关论文
共 43 条
[1]
Oxazolidinone antibiotics target the P site on Escherichia coli ribosomes
[J].
Aoki, H
;
Ke, LZ
;
Poppe, SM
;
Poel, TJ
;
Weaver, EA
;
Gadwood, RC
;
Thomas, RC
;
Shinabarger, DL
;
Ganoza, MC
.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,
2002, 46 (04)
:1080-1085

Aoki, H
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Ke, LZ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Poppe, SM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Poel, TJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Weaver, EA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Gadwood, RC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Thomas, RC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Shinabarger, DL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

Ganoza, MC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada
[2]
A genome-based approach for the identification of essential bacterial genes
[J].
Arigoni, F
;
Talabot, F
;
Peitsch, M
;
Edgerton, MD
;
Meldrum, E
;
Allet, E
;
Fish, R
;
Jamotte, T
;
Curchod, ML
;
Loferer, H
.
NATURE BIOTECHNOLOGY,
1998, 16 (09)
:851-856

Arigoni, F
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Talabot, F
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Peitsch, M
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Edgerton, MD
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Meldrum, E
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Allet, E
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Fish, R
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Jamotte, T
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Curchod, ML
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland

Loferer, H
论文数: 0 引用数: 0
h-index: 0
机构:
Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland Glaxo Wellcome Res & Dev Ltd, Geneva Biomed Res Inst, Geneva, Switzerland
[3]
Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis:: Conditional complementation of a teichoic acid mutant
[J].
Bhavsar, AP
;
Zhao, XM
;
Brown, ED
.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,
2001, 67 (01)
:403-410

Bhavsar, AP
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada

Zhao, XM
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada

Brown, ED
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada McMaster Univ, Dept Biochem, Antimicrobial Res Ctr, Hamilton, ON L8N 3Z5, Canada
[4]
Catalytic properties of mutant 23 S ribosomes resistant to oxazolidinones
[J].
Bobkova, EV
;
Yan, YP
;
Jordan, DB
;
Kurilla, MG
;
Pompliano, DL
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2003, 278 (11)
:9802-9807

Bobkova, EV
论文数: 0 引用数: 0
h-index: 0
机构:
Bristol Myers Squibb Co, Wilmington, DE 19880 USA Bristol Myers Squibb Co, Wilmington, DE 19880 USA

Yan, YP
论文数: 0 引用数: 0
h-index: 0
机构:
Bristol Myers Squibb Co, Wilmington, DE 19880 USA Bristol Myers Squibb Co, Wilmington, DE 19880 USA

Jordan, DB
论文数: 0 引用数: 0
h-index: 0
机构:
Bristol Myers Squibb Co, Wilmington, DE 19880 USA Bristol Myers Squibb Co, Wilmington, DE 19880 USA

Kurilla, MG
论文数: 0 引用数: 0
h-index: 0
机构:
Bristol Myers Squibb Co, Wilmington, DE 19880 USA Bristol Myers Squibb Co, Wilmington, DE 19880 USA

Pompliano, DL
论文数: 0 引用数: 0
h-index: 0
机构:
Bristol Myers Squibb Co, Wilmington, DE 19880 USA Bristol Myers Squibb Co, Wilmington, DE 19880 USA
[5]
THE GTPASE SUPERFAMILY - A CONSERVED SWITCH FOR DIVERSE CELL FUNCTIONS
[J].
BOURNE, HR
;
SANDERS, DA
;
MCCORMICK, F
.
NATURE,
1990, 348 (6297)
:125-132

BOURNE, HR
论文数: 0 引用数: 0
h-index: 0
机构: UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA

SANDERS, DA
论文数: 0 引用数: 0
h-index: 0
机构: UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA

MCCORMICK, F
论文数: 0 引用数: 0
h-index: 0
机构: UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA
[6]
Cell cycle arrest in era GTPase mutants:: a potential growth rate-regulated checkpoint in Escherichia coli
[J].
Britton, RA
;
Powell, BS
;
Dasgupta, S
;
Sun, Q
;
Margolin, W
;
Lupski, JR
;
Court, DL
.
MOLECULAR MICROBIOLOGY,
1998, 27 (04)
:739-750

Britton, RA
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Powell, BS
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Dasgupta, S
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Sun, Q
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Margolin, W
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Lupski, JR
论文数: 0 引用数: 0
h-index: 0
机构: NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA

Court, DL
论文数: 0 引用数: 0
h-index: 0
机构:
NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Lab Gene Regulat & Chromosome Biol, Frederick, MD 21702 USA
[7]
MURA (MURZ), THE ENZYME THAT CATALYZES THE FIRST COMMITTED STEP IN PEPTIDOGLYCAN BIOSYNTHESIS, IS ESSENTIAL IN ESCHERICHIA-COLI
[J].
BROWN, ED
;
VIVAS, EI
;
WALSH, CT
;
KOLTER, R
.
JOURNAL OF BACTERIOLOGY,
1995, 177 (14)
:4194-4197

BROWN, ED
论文数: 0 引用数: 0
h-index: 0
机构: HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA

VIVAS, EI
论文数: 0 引用数: 0
h-index: 0
机构: HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA

WALSH, CT
论文数: 0 引用数: 0
h-index: 0
机构: HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA

KOLTER, R
论文数: 0 引用数: 0
h-index: 0
机构: HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA
[8]
RNA methylation under heat shock control
[J].
Bügl, H
;
Fauman, EB
;
Staker, BL
;
Zheng, FH
;
Kushner, SR
;
Saper, MA
;
Bardwell, JCA
;
Jakob, U
.
MOLECULAR CELL,
2000, 6 (02)
:349-360

Bügl, H
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Fauman, EB
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Staker, BL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Zheng, FH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Kushner, SR
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Saper, MA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Bardwell, JCA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

Jakob, U
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA
[9]
Structural and biochemical analysis of the Obg GTP binding protein
[J].
Buglino, J
;
Shen, V
;
Hakimian, P
;
Lima, CD
.
STRUCTURE,
2002, 10 (11)
:1581-1592

Buglino, J
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Shen, V
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Hakimian, P
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Lima, CD
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA
[10]
Function of the universally conserved bacterial GTPases
[J].
Caldon, CE
;
March, PE
.
CURRENT OPINION IN MICROBIOLOGY,
2003, 6 (02)
:135-139

Caldon, CE
论文数: 0 引用数: 0
h-index: 0
机构:
Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

March, PE
论文数: 0 引用数: 0
h-index: 0
机构:
Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia