Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

被引：39

作者：

Costa, Leandro Silva ^{[1
,2
]}

Silva Telles, Cinthia Beatrice ^{[1
]}

Oliveira, Ruth Medeiros ^{[1
]}

Duarte Barreto Nobre, Leonardo Thiago ^{[1
]}

Dantas-Santos, Nednaldo ^{[1
]}

Gomes Camara, Rafael Barros ^{[1
]}

Santos Pereira Costa, Mariana Santana ^{[1
]}

Almeida-Lima, Jailma ^{[1
]}

Melo-Silveira, Raniere Fagundes ^{[1
]}

Lopes Albuquerque, Ivan Rui ^{[1
]}

Leite, Edda Lisboa ^{[1
]}

Oliveira Rocha, Hugo Alexandre ^{[1
]}

机构：

[1] Univ Fed Rio Grande Norte UFRN, Lab Biotechnol Nat Polymers BIOPOL, Dept Biochem, Natal, RN, Brazil

[2] Fed Inst Educ Sci & Technol Rio Grande Norte IFRN, Santa Cruz, RN, Brazil

来源：

MARINE DRUGS | 2011年 / 9卷 / 04期

关键词：

fucoidan; sulfated polysaccharides; anticancer; apoptosis-inducing factor (AIF); ANTITUMOR-ACTIVITY; FUCOIDAN; ANTICOAGULANT; INHIBITION; ACTIVATION; CASPASE-3;

D O I：

10.3390/md9040603

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated L-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NF kappa B were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.

引用

页码：603 / 614

页数：12

共 24 条

[1]

Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways [J].