Derivation of dihedral angles from CH-CH dipolar-dipolar cross-correlated relaxation rates:: A C-C torsion involving a quaternary carbon atom in epothilone A bound to tubulin

被引：34

作者：

Carlomagno, T

Sánchez, VM

Blommers, MJJ

Griesinger, C

机构：

[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[2] Novartis Pharma AG, CH-4002 Basel, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 22期

关键词：

conformation analysis; dihedral angle; natural products; NMR spectroscopy; tubulin;

D O I：

10.1002/anie.200350950

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

C-C torsion angles in HC-CCH3 moieties can be measured by a new NMR experiment based on the measurement of CH-CH dipolar-dipolar cross-correlated relaxation rates and is demonstrated on a mixture of 13C-labeled epothilone (1) and tubulin. The new pulse sequence is broadly applicable to any HC-CCH3 moiety and therefore to a number of amino acid side chains in proteins.

引用

收藏

页码：2515 / 2517

页数：3

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