Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex

被引：28

作者：

Kim, H ^{[1
]}

von Heijne, G ^{[1
]}

Nilsson, I ^{[1
]}

机构：

[1] Stockholm Univ, Dept Biochem & Biophys, SE-10691 Stockholm, Sweden

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2005年 / 280卷 / 21期

关键词：

D O I：

10.1074/jbc.M412213200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The highly conserved membrane protein STT3 is part of the oligosaccharyl transferase complex in the endoplasmic reticulum of eukaryotic cells. Various experimental observations strongly suggest that STT3 contains the active site of the complex. Here, we report a detailed topology study of STT3 from two different organisms, Saccharomyces cerevisiae and mouse, using in vivo and in vitro topology mapping assays. Our results suggest that STT3 has 11 transmembrane helices and an overall N-cyt-C-lum orientation.

引用

页码：20261 / 20267

页数：7

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