Multi-Lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers

被引：44

作者：

Ali, Hamad ^{[1
,2
]}

Al-Yatama, Majda K. ^{[3
]}

Abu-Farha, Mohamed ^{[4
]}

Behbehani, Kazem ^{[1
]}

Al Madhoun, Ashraf ^{[1
]}

机构：

[1] Dasman Diabet Inst, Dept Basic Sci Res, Dasman 1180, Kuwait

[2] Kuwait Univ, Fac Allied Hlth Sci, Hlth Sci Ctr, Dept Med Lab Sci MLS, Kuwait, Kuwait

[3] Dar Al Baraa Med Ctr, Hawally 3357, Kuwait

[4] Dasman Diabet Inst, Biochem & Mol Biol Unit, Dasman 1180, Kuwait

来源：

PLOS ONE | 2015年 / 10卷 / 04期

关键词：

BONE-MARROW; PROLIFERATION; SUPPRESSION; PHENOTYPE; MIGRATION; PROTEOMES; EXPANSION; RESPONSES; RECEPTOR; CAPACITY;

D O I：

10.1371/journal.pone.0122465

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Wharton's Jelly-derived Mesenchymal stem cells (WJ-MSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal, differentiation and unique immunomodulatory properties. Although many studies have characterized various WJ-MSCs biologically, the expression profiles of the commonly used stemness markers have not yet been addressed. In this study, WJ-MSCs were isolated and characterized for stemness and surface markers expression. Flow cytometry, immunofluorescence and qRT-PCR analysis revealed predominant expression of CD29, CD44, CD73, CD90, CD105 and CD166 in WJ-MSCs, while the hematopoietic and endothelial markers were absent. Differential expression of CD 29, CD90, CD105 and CD166 following adipogenic, osteogenic and chondrogenic induction was observed. Furthermore, our results demonstrated a reduction in CD44 and CD73 expressions in response to the tri-lineage differentiation induction, suggesting that they can be used as reliable stemness markers, since their expression was associated with undifferentiated WJ-MSCs only.

引用

页数：18

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