Miniature postsynaptic currents depend on Ca2+ released from internal stores via PLC/IP3 pathway

被引：22

作者：

Han, MH ^{[1
]}

Kawasaki, A ^{[1
]}

Wei, JY ^{[1
]}

Barnstable, CJ ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Ophthalmol & Visual Sci, New Haven, CT 06520 USA

来源：

NEUROREPORT | 2001年 / 12卷 / 10期

关键词：

Ca2+; internal stores; IP3; miniature; neurotransmitter release;

D O I：

10.1097/00001756-200107200-00032

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Miniature postsynaptic currents (mPSCs) were examined on autaptic innervation of single rat retinal ganglion cells in low density cultures. Removal of Ca2+ from bath solution or blocking of Ca2+ channels by Cd2+ had no detectable effect on mPSC frequency or amplitude. Thapsigargin, an agent for mobilization of Ca2+ from internal stores, increased mPSC frequency 3-5-fold in control, Ca2+-free or Cd2+-containing solutions. The inositol 1,4,5-triphosphate (IP3) receptor antagonist, heparin; the phospholipase C (PLC) inhibitor, U73 122; and caffeine abolished mPSC or decreased mPSCs frequency. Calcium imaging showed that cytosolic Ca2+ was increased by thapsigargin and decreased by caffeine. These data demonstrate that internal store-released Ca2+ regulated by the PLC/IP3/IP3-receptor pathway has critical contribution to generation and control of miniature release in retinal ganglion cells. NeuroReport 12:2203-2207 (C) 2001 Lippincott Williams & Wilkins.

引用

页码：2203 / 2207

页数：5

共 25 条

[21] Pharmacological analysis of intracellular Ca2+ signalling:: problems and pitfalls
Taylor, CW
Broad, LM
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1998, 19 (09) : 370 - 375
[22] CGMP inhibits IP3-induced Ca2+ release in intact rat megakaryocytes via cGMP- and cAMP-dependent protein kinases
Tertyshnikova, X
Yan, XW
Fein, A
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1998, 512 (01): : 89 - 96
[23] TOESCU EC, 1992, J BIOL CHEM, V267, P23467
[24] Yoshihara M, 1999, J NEUROSCI, V19, P2432
[25] Zhang CL, 1999, J NEUROSCI, V19, P2852

← 1 2 3 →