DNA gyrase gene in Neisseria gonorrhoeae as indicator for resistance to ciprofloxacin and species verification

被引:14
作者
Lindbäck, E
Gharizadeh, B
Ataker, F
Airell, Å
Jalal, S
Nyrén, P
Wretlind, B
机构
[1] F72 Huddinge Univ Hosp, Dept Clin Bacteriol, Div Lab Med, SE-14186 Stockholm, Sweden
[2] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA
[3] Royal Inst Technol, Stockholm Ctr Phys Astron & Biotechnol, Dept Biotechnol, SE-10691 Stockholm, Sweden
关键词
Neisseria gonorrhoeae; QRDR; gyrA; ciprofloxacin; Pyrosequencing technology; antibiotic susceptibility;
D O I
10.1258/0956462053057675
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have identified a unique region of eight amino acids in the quinolone resistance-determining region in the gyrA gene in Neisseria gonorrhoeae as an indicator of resistance to fluoroquinolones. We sequenced that region by the Pyrosequencing(TM) technology in 46 N. gonorrhoeae strains and 11 urine samples positive in AMPLICOR(TM) N. gonorrhoeae polymerase chain reaction (Roche Diagnostics), with corresponding isolates of N. gonorrhoeae. The results showed that 28 samples with minimum inhibitory concentration (MIC) of ciprofloxacin > 1 mg/L had mutations in positions 91 and 95. Fifteen samples with MIC 0.125-1.0 mg/L had either one or both of the mutations. The 14 susceptible samples had no mutations. The target region also discriminates N. gonorrhoeae from other species of Neisseria. Our conclusion is that gyrA is an indicator of resistance to ciprofloxacin in N. gonorrhoeae and sequencing by Pyrosequencing technology is a suitable tool for analysis of DNA in urine samples.
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收藏
页码:142 / 147
页数:6
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