Localization of BCR-ABL to F-actin regulates cell adhesion but does not attenuate CML development

We have previously found that P210(BCR-ABL) increases the adhesion of hematopoietic cell lines to fibronectin by a mechanism that is independent of tyrosine kinase activity. To investigate the pathway(s) by which P210(BCR-ABL) influences cell adhesion, we used a quantitative cell adhesion device that can discern small changes in cell adhesion to assay P210(BCR-ABL) with mutations in several critical domains. We expressed P210(BCR-ABL) mutants in 32D myeloblast cells and found that binding to fibronectin is mediated primarily by the alpha(5)beta(1) integrin. We performed a structure/function analysis to map domains important for cell adhesion. Increased adhesion was mediated by 3 domains: (1) the N-terminal coiled-coil domain that facilitates oligomerization and F-actin localization; (2) bcr sequences between aa 163 to 210; and (3) F-actin localization through the C-terminal actin-binding domain of c-abl. We compared our adhesion results with the ability of these mutants to cause a chronic myelogenous leukemia (CML)-like disease in a murine bone marrow transplantation assay and found that adhesion to fibronectin did not correlate with the ability of these mutants to cause CML. Together, our results suggest that F-actin localization may play a pivotal, role in modulating adhesion but that it is dispensable for the development (C) 2003 by The American Society of Hematology.