B cell immunosenescence: different features of naive and memory B cells in elderly

被引：76

作者：

Buffa, Silvio ^{[1
]}

Bulati, Matteo ^{[1
]}

Pellicano, Mariavaleria ^{[2
]}

Dunn-Walters, Deborah K. ^{[3
]}

Wu, Yu-Chang ^{[3
]}

Candore, Giuseppina ^{[1
]}

Vitello, Salvatore ^{[4
,5
]}

Caruso, Calogero ^{[1
]}

Colonna-Romano, Giuseppina ^{[1
]}

机构：

[1] Univ Palermo, Immunosenescence Unit, Dept Pathobiol & Med & Forens Biotechnol, I-90134 Palermo, Italy

[2] Univ Tubingen, Sch Med, Med Res Ctr, Tubingen, Germany

[3] Kings Coll London, Sch Med, Dept Immunobiol, London WC2R 2LS, England

[4] Dipartimentale Anziani, Dipartimento Cure Primarie Serv AUSL6, Palermo, Italy

[5] ADI, Palermo, Italy

来源：

BIOGERONTOLOGY | 2011年 / 12卷 / 05期

关键词：

Cytokines; Elderly; Hypermutation; Inflammation; Memory B cells; IMMUNE-RESPONSE; CLASS SWITCH; IGM MEMORY; POPULATION; AGE; CYTOMEGALOVIRUS; COMPARTMENT; VACCINATION; REPERTOIRE; PROFILES;

D O I：

10.1007/s10522-011-9353-4

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Elderly people show a reduced protection against new infections and a decreased response to vaccines as a consequence of impairment of both cellular and humoral immunity. In this paper we have studied memory/na < ve B cells in the elderly, evaluating surface immunoglobulin expression, production of the pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-alpha and interleukin (IL)-10, and presence of somatic hypermutation, focusing on the IgG(+)IgD(-)CD27(-) double negative (DN) B cells that are expanded in the elderly. Our results show that na < ve B cells from young donors need a sufficiently strong stimulus to be activated "in vitro", while na < ve B cells from old subjects are able to produce IL-10 and TNF-alpha when stimulated "physiologically" (alpha-CD40/IL-4), suggesting that these cells might play a role in the control of the immuno-inflammatory environment in the elderly. In addition, in the elderly there is an accumulation of DN B cells with a reduced rate of somatic hypermutation. Thus, DN B lymphocytes may be exhausted cells that are expanded and accumulate as a by-product of persistent stimulation or impaired germinal center formation.

引用

页码：473 / 483

页数：11

共 47 条

[1] The human immunomodulatory CD25+ B cell population belongs to the memory B cell pool [J].