Optimisation of medium composition for clavulanic acid production by Streptomyces clavuligerus

被引：52

作者：

Gouveia, ER ^{[1
]}

Baptista-Neto, A ^{[1
]}

Badino, AC ^{[1
]}

Hokka, CO ^{[1
]}

机构：

[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP, Brazil

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

clavulanic acid; empirical model; experimental design; medium composition; Streptomyces clavuligerus;

D O I：

10.1023/A:1010356231673

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Among four different commercially available nitrogen sources containing soybean derivatives, a protein extract of soybean gave the highest yield for clavulanic acid production by Streptomyces clavuligerus. A statistical method based on factorial design of experiments was applied to optimise the medium. An empirical model was obtained by applying response surface statistical analysis. The analysis of variance showed that concentrations of protein extract of soybean and glycerol and the interaction between these two variables were significant at 95% level of confidence. The maximum clavulanic acid concentration obtained in 72 h was 1.2 g l(-1).

引用

页码：157 / 161

页数：5

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