Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies

被引：70

作者：

Cordwell, Stuart J. ^{[1
,2
]}

Alice, C. L. Len ^{[3
]}

Touma, Rachel G. ^{[1
]}

Scott, Nichollas E. ^{[1
]}

Falconer, Linda ^{[4
]}

Jones, David ^{[5
]}

Connolly, Angela ^{[1
]}

Ben Crossett ^{[1
]}

Djordjevic, Steven P. ^{[4
]}

机构：

[1] Univ Sydney, Sch Mol & Microbial Biosci, Sydney, NSW 2006, Australia

[2] Univ Sydney, Discipline Pathol, Sydney, NSW 2006, Australia

[3] Australian Proteome Anal Facil, Sydney, NSW, Australia

[4] Elizabeth Macarthur Agr Inst, NSW Dept Primary Ind, Camden, NSW, Australia

[5] Royal Hobart Hosp, Dept Microbiol, Hobart, Tas, Australia

来源：

PROTEOMICS | 2008年 / 8卷 / 01期

关键词：

mass spectrometry; microbial proteomics; outer membrane proteins; two-dimensional gel electrophoresis; two-dimensional liquid chromatography;

D O I：

10.1002/pmic.200700561

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Campylobacter jejuni is the leading cause of food- and water-borne illness world-wide. The membrane-associated proteome of a recent C. jejuni gastrointestinal isolate (JHH1) was generated by sodium carbonate precipitation and ultracentrifugation followed by 2-DE and MALDI-TOF MS as well as 2-DLC (strong cation exchange followed by RP chromatography) of trypsin digests coupled to MS/MS (2-DLC/MS/MS). 2-DE/MS identified 77 proteins, 44 of which were predicted membrane proteins, while 2-DLC/MS/MS identified 432 proteins, of which 206 were predicted to be membrane associated. A total of 453 unique proteins (27.4% of the C. jejuni theoretical proteome), including 187 bonafide membrane proteins were identified in this study. Membrane proteins were also compared between C. jejuni JHH1 and ATCC 700297 to identify factors potentially associated with increased gastrointestinal virulence. We identified 28 proteins that were significantly (>two-fold) more abundant in, or unique to, JHH1, including eight proteins involved in chemotaxis signal transduction and flagellar motility the amino acid-binding surface antigens CjaA and CjaC, and four outer membrane proteins (OMPs) of unknown function (Cj0129c, Cj1031, Cj1279c, and Cj1721c). Immunoblotting using convalescent patient sera generated postgastrointestinal infection revealed 13 (JHH1) and 12 (ATCC 700297) immunoreactive proteins. These included flagellin (FlaA) and CadF as well as Omp18, Omp50, Cj1721c, PEB1A, PEB2, and PEB4A. This study provides a comprehensive analysis of membrane associated proteins from C. jejuni.

引用

页码：122 / 139

页数：18

共 62 条

[21] JlpA of Campylobacter jejuni interacts with surface-exposed heat shock protein 90α and triggers signalling pathways leading to the activation of NF-κB and p38 MAP kinase in epithelial cells [J].

Jin, SM ;

Song, YC ;

Emili, A ;

Sherman, PM ;

Chan, VL .

CELLULAR MICROBIOLOGY, 2003, 5 (03) :165-174

[22] JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells [J].

Jin, SM ;

Joe, A ;

Lynett, J ;

Hani, EK ;

Sherman, P ;

Chan, VL .

MOLECULAR MICROBIOLOGY, 2001, 39 (05) :1225-1236

[23] Adaptation of Campylobacter jejuni NCTC11168 to high-level colonization of the avian gastrointestinal tract [J].

Jones, MA ;

Marston, KL ;

Woodall, CA ;

Maskell, DJ ;

Linton, D ;

Karlyshev, AV ;

Dorrell, N ;

Wren, BW ;

Barrow, PA .

INFECTION AND IMMUNITY, 2004, 72 (07) :3769-3776

[24] The Campylobacter jejuni glycome [J].

Karlyshev, AV ;

Ketley, JM ;

Wren, BW .

FEMS MICROBIOLOGY REVIEWS, 2005, 29 (02) :377-390

[25] ISOLATION AND CHARACTERIZATION OF 2 CAMPYLOBACTER GLYCINE-EXTRACTED PROTEINS THAT BIND TO HELA-CELL MEMBRANES [J].

KERVELLA, M ;

PAGES, JM ;

PEI, ZH ;

GROLLIER, G ;

BLASER, MJ ;

FAUCHERE, JL .

INFECTION AND IMMUNITY, 1993, 61 (08) :3440-3448

[26] Identification of a fibronectin-binding domain within the Campylobacter jejuni CadF protein [J].

Konkel, ME ;

Christensen, JE ;

Keech, AM ;

Monteville, MR ;

Klena, JD ;

Garvis, SG .

MOLECULAR MICROBIOLOGY, 2005, 57 (04) :1022-1035

[27] Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins [J].

Konkel, ME ;

Mead, DJ ;

Cieplak, W .

INFECTION AND IMMUNITY, 1996, 64 (05) :1850-1853

[28] Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni [J].

Konkel, ME ;

Garvis, SG ;

Tipton, SL ;

Anderson, DE ;

Cieplak, W .

MOLECULAR MICROBIOLOGY, 1997, 24 (05) :953-963

[29] The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids [J].

Leon-Kempis, Maria del Rocio ;

Guccione, Edward ;

Mulholland, Francis ;

Williamson, Michael P. ;

Kelly, David J. .

MOLECULAR MICROBIOLOGY, 2006, 60 (05) :1262-1275

[30] CmeABC functions as a multidrug efflux system in Campylobacter jejuni [J].

Lin, J ;

Michel, LO ;

Zhang, QJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (07) :2124-2131

← 1 2 3 4 5 6 7 →