The cholinergic gene locus in amphioxus: Molecular characterization and developmental expression patterns

被引：13

作者：

Candiani, Simona ^{[1
]}

Lacalli, Thurston C. ^{[2
]}

Parodi, Manuela ^{[1
]}

Oliveri, Diana ^{[1
]}

Pestarino, Mario ^{[1
]}

机构：

[1] Univ Genoa, Dept Biol, I-16132 Genoa, Italy

[2] Univ Victoria, Dept Biol, Victoria, BC V8W 2Y2, Canada

来源：

DEVELOPMENTAL DYNAMICS | 2008年 / 237卷 / 05期

关键词：

amphioxus; cholinergic motoneurons and interneurons; cholinergic gene locus; ChAT; VAChT; development; evolution; ISH;

D O I：

10.1002/dvdy.21541

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The cholinergic gene locus (CGL), consisting of the vesicular acetylcholine transporter (VAChT)/choline acetyltransferase (ChAT) gene, encodes two specific cholinergic neuronal markers used extensively to study cholinergic transmission. In the present work, we isolated the amphioxus homologs of VAChT and ChAT and examined their expression during development. Analysis of the 5' untranslated region of VAChT and ChAT suggests that the splicing of the VAChT/ChAT mRNA has been evolutionarily conserved in amphioxus and mammals. By double whole-mount in situ hybridization, we demonstrate that VAChT and ChAT are coexpressed in the same cells. They are first expressed in four pairs of differentiating cells in the neural plate. Their later expression is primarily in the anterior nerve cord in several types of motoneurons, some of the interneurons and in the receptor cells of the larval ocellus.

引用

收藏

页码：1399 / 1411

页数：13

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