Cardiolipin synthase of Arabidopsis thaliana

被引：42

作者：

Nowicki, M ^{[1
]}

Müller, F ^{[1
]}

Frentzen, M ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Inst Biol 1, D-52056 Aachen, Germany

来源：

FEBS LETTERS | 2005年 / 579卷 / 10期

关键词：

cytidinediphosphate-diacylglycerol; cardiolipin synthase; functional expression; mitochondria; phospholipid synthesis; Arabidopsis thaliana;

D O I：

10.1016/j.febslet.2005.03.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K. for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V.. All rights reserved.

引用

页码：2161 / 2165

页数：5

共 34 条

[11] Cardiolipin is not essential for the growth of Saccharomyces cerevisiae on fermentable or non-fermentable carbon sources [J].

Jiang, F ;

Rizavi, HS ;

Greenberg, ML .

MOLECULAR MICROBIOLOGY, 1997, 26 (03) :481-491

[12] Cardiolipin synthase expression is essential for growth at elevated temperature and is regulated by factors affecting mitochondrial development [J].

Jiang, F ;

Gu, ZM ;

Granger, JM ;

Greenberg, ML .

MOLECULAR MICROBIOLOGY, 1999, 31 (01) :373-379

[13] Absence of cardiolipin in the crd1 null mutant results in decreased mitochondrial membrane potential and reduced mitochondrial function [J].

Jiang, F ;

Ryan, MT ;

Schlame, M ;

Zhao, M ;

Gu, ZM ;

Klingenberg, M ;

Pfanner, N ;

Greenberg, ML .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (29) :22387-22394

[14] Identification of an Arabidopsis thaliana gene for cardiolipin synthase located in mitochondria [J].

Katayama, K ;

Sakurai, I ;

Wada, H .

FEBS LETTERS, 2004, 577 (1-2) :193-198

[15] Isolation of a Chinese hamster ovary (CHO) cDNA encoding phosphatidylglycerophosphate (PGP) synthase, expression of which corrects the mitochondrial abnormalities of a PGP synthase-defective mutant of CHO-K1 cells [J].

Kawasaki, K ;

Kuge, O ;

Chang, SC ;

Heacock, PN ;

Rho, M ;

Suzuki, K ;

Nishijima, M ;

Dowhan, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (03) :1828-1834

[16] Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes [J].

Krogh, A ;

Larsson, B ;

von Heijne, G ;

Sonnhammer, ELL .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (03) :567-580

[17] SUBCELLULAR AND SUBMITOCHONDRIAL LOCALIZATION OF PHOSPHOLIPID-SYNTHESIZING ENZYMES IN SACCHAROMYCES-CEREVISIAE [J].

KUCHLER, K ;

DAUM, G ;

PALTAUF, F .

JOURNAL OF BACTERIOLOGY, 1986, 165 (03) :901-910

[18] Phosphatidylglycerophosphate synthases from Arabidopsis thaliana [J].

Müller, F ;

Frentzen, M .

FEBS LETTERS, 2001, 509 (02) :298-302

[19] Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites [J].

Nielsen, H ;

Engelbrecht, J ;

Brunak, S ;

vonHeijne, G .

PROTEIN ENGINEERING, 1997, 10 (01) :1-6

[20] Lack of mitochondrial anionic phospholipids causes an inhibition of translation of protein components of the electron transport chain - A yeast genetic model system for the study of anionic phospholipid function in mitochondria [J].

Ostrander, DB ;

Zhang, M ;

Mileykovskaya, E ;

Rho, M ;

Dowhan, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) :25262-25272

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