Development of a sandwich ELISA for ovine granulocyte macrophage colony-stimulating factor

被引：36

作者：

Entrican, G

Deane, D

MacLean, M

Inglis, L

Thomson, J

McInnes, C

Haig, DM

机构：

[1] Moredun Research Institute, Edinburgh EH17 7JH

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1996年 / 50卷 / 1-2期

关键词：

granulocyte/macrophage colony-stimulating factor; sandwich ELISA; ovine; haemopoiesis;

D O I：

10.1016/0165-2427(95)05468-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recombinant ovine granulocyte/macrophage colony-stimulating factor (rOv GM-CSF) has been expressed in Chinese hamster ovary cells. A stable, cloned line of these cells has been established which secretes high levels (40 mu g ml(-1)) of rOv GM-CSF. Three murine monoclonal antibodies (mAbs) were produced which reacted with rOv GM-CSF on Western blots. These mAbs also neutralised the activity of both recombinant and native Ov GM-CSF in a bone marrow haemopoietic progenitor cell assay. Two of the mAbs, which recognise mutually exclusive epitopes, were selected for the development of a sandwich enzyme-linked immunosorbent assay (ELISA) to measure GM-CSF in biological samples of ovine origin.

引用

页码：105 / 115

页数：11

共 21 条

[1] PARAMETERS AFFECTING ASCITES TUMOR-FORMATION IN MICE AND MONOCLONAL-ANTIBODY PRODUCTION [J].