Host inflammatory response profiling in preeclampsia using an in vitro whole blood stimulation model

被引：34

作者：

Brewster, J. A. ^{[1
]}

Orsi, N. M. ^{[1
]}

Gopichandran, N. ^{[1
]}

Ekbote, U. V. ^{[1
]}

Cadogan, E. ^{[1
]}

Walker, J. J. ^{[1
]}

机构：

[1] St James Univ Hosp, Leeds Inst Mol Med, Liz Dawn Pathol & Translat Sci Ctr, Perinatal Res Grp, Leeds LS9 7TF, W Yorkshire, England

来源：

HYPERTENSION IN PREGNANCY | 2008年 / 27卷 / 01期

关键词：

cytokines; lipopolysaccharide; in vitro; whole blood;

D O I：

10.1080/10641950701826067

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The pathophysiology of preeclampsia (PET) implicates an inflammatory dysfunction. This study profiled this host response by challenging whole blood with lipopolysaccharide. Multiplex immunoassays determined interleukin (IL)-1 beta IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17, granulocyte/granulocyte macrophage-colony stimulating factors (G-CSF/GM-SCF), interferon(IFN)-gamma monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein-1 beta and tumor necrosis factor (TNF)-alpha levels. Secretory capacity was expressed in pg/million white cells or monocytes (SEM). PET featured significantly higher IL-1, IL-2, IL-10, IL-13, G-CSF, IFN-gamma MCP-1 and TNF-alpha monocyte secretory capacities (p < 0.05). The PET group exhibited an inflammatory hyper-responsiveness (p < 0.01) which was poorly described by the traditional Th1:Th2 dichotomy.

引用

页码：1 / 16

页数：16

共 47 条

[1] Differential levels of interleukin 6 in maternal and cord sera and placenta in women with pre-eclampsia [J].