A complementation of two genes originating from susceptible Capsicum annuum lines confers a new and complete resistance to pepper veinal mottle virus

A doubled haploid (DH) line completely resistant to pepper veinal mottle virus (PVMV) was recovered from the F-1 hybrid between two Capsicum annuum lines, Perennial and Florida VR2, whereas both of these lines were susceptible to PVMV. These observations suggest that resistance to PVMV results from a complementary action of genes originating from Perennial and Florida VR2. F-2 plants were obtained by crossing individual DH lines from a hybrid (Perennial x Yolo Wonder) with Florida VR2. In 14 F-2 families, the segregation ratios observed for PVMV resistance were consistent with the complementation of two recessive genes, each one coming from one of the parents. One gene from Florida VR2 was identical or tightly linked to pvr2(2) (a recessive gene conferring complete resistance to potato virus Y [PVY] pathotypes [0 and 1]). The other gene from Perennial was linked to a restriction fragment length polymorphism marker localized on chromosome Pourpre of the pepper map and was tentatively named pvr6. The pvr2(2) gene alone conferred complete resistance to PVY(0) and (1), and in the genomic region around the PVMV-linked marker, we previously mapped genes also involved in potyvirus resistance. Complementation between factors already involved in potyvirus resistance conferred an enlarged spectrum of resistance to a distantly related pathogen.