An optical technique for remote focusing in microscopy

被引：178

作者：

Botcherby, E. J. ^{[1
]}

Juskaitis, R. ^{[1
]}

Booth, M. J. ^{[1
]}

Wilson, T. ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

OPTICS COMMUNICATIONS | 2008年 / 281卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

sectioning microscopy; high numerical aperture theory;

D O I：

10.1016/j.optcom.2007.10.007

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe the theory of a new method of optical refocusing that is particularly relevant for confocal and multiphoton microscopy systems. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free refocusing can be achieved over an axial scan range of 70 mu m for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method enables high axial scan speeds without mechanical interference between the objective lens and the specimen. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：880 / 887

页数：8

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