Diabetic modulation of the temperature kinetics properties of cytochrome oxidase activity in rat brain mitochondria

The effects of alloxan-diabetes and subsequent treatment with insulin on temperature kinetics properties of cytochrome oxidase activity from rat brain mitochondria were examined. The enzyme activity decreased only at the late stage of diabetes which was not normalized by insulin treatment; however at early stage of diabetes hyper-stimulation occurred. In the control animals the Arrhenius plot was chair shaped with three energies of (E-1, E-2 and E-3) and two phase transition temperatures (T-t1 and T-t2). At early diabetic stage the Arrhenius plot became biphasic and E-1 and E-2 decreased(;) insulin treatment reversed chair-shaped pattern with increase in E-2. These changes correlated with transient changes in the phospholipids profiles especially decreased acidic phospholipids. The temperature kinetics parameters were minimally affected at the late stage of diabetes or by insulin treatment. Thus at the late stage the brain tissue seems to have readjusted to its insulin homeostasis.