Application of a PDGF-containing novel gel for cutaneous wound healing

Aim: The objective of this study was to develop an acellular provisional matrix to mimic the extracellular matrix (ECM) of a wound site, and to evaluate its potential for in vivo dermal regeneration. Key points: This study evaluates the potential of a monomeric collagen-chitosan gel containing platelet-derived growth factor to enhance healing and reduce the time of wound closure. Main methods: In the present study, we developed a novel composite consisting of the combination of collagen, chitosan and platelet-derived growth factor (PDGF). This biodegradable Molecular gel matrix was used as a biomimetic surface for cell attachment to promote healing of excision wounds (1.5 cm long x 1.5 cm width). The rate of wound contraction and the concentrations of collagen, hexosamine, uronic acid and growth factors in the granulation tissue were determined. The amount of antioxidants and lipid peroxide in the treated tissues was elevated compared to the control tissue; in particular, tissue from the group treated with the collagen-chitosan-PDGF matrix had remarkable increase in these parameters. Additionally, the histology and ultrastructure of re-epithelialized tissues at the wound site were recorded. Significance: This study reveals that the monomeric nature of biological molecules in association with PDGF exerted simultaneous biomimetic and chemotactic effects and promoted wound healing. (C) 2010 Elsevier Inc. All rights reserved.