Cullin-5 is ubiquitous in the rat brain

被引：8

作者：

Ceremuga, TE

Yao, XL

McCabe, JT

机构：

[1] Uniformed Serv Univ Hlth Sci, F Edward Hebert Sch Med, Grad Program Neurosci, Bethesda, MD 20814 USA

[2] Uniformed Serv Univ Hlth Sci, F Edward Hebert Sch Med, Dept Anat Physiol & Genet, Bethesda, MD 20814 USA

来源：

NEUROSCIENCE LETTERS | 2003年 / 345卷 / 02期

关键词：

cullin; cullin-5; gene expression; immunocytochemistry; ubiquitin ligase; proteasome;

D O I：

10.1016/S0304-3940(03)00298-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cullin-5 (Cul-5), an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome, was examined for its localization and distribution in the rat central nervous system (CNS). We showed cul-5 mRNA expression in rat neuronal, glial, and vascular endothelial cells by reverse transcription-polymerase chain reaction and corroborated these data by Cul-5 immunostaining in neurons, astrocytes, blood vessels, and choroid plexus of the laboratory rat. Widespread and ubiquitous expression of Cul-5 in the brain suggests that it may have a vital role(s) in cellular activities of the CNS. (C) 2003 Published by Elsevier Science Ireland Ltd.

引用

页码：121 / 125

页数：5

共 14 条

[11] MUF1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase [J].