Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution

被引：6

作者：

Ge, Jing

Liu, Zhaofang

Zhi, Zeyong

Zhao, Xin Sheng ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 413卷 / 01期

基金：

中国国家自然科学基金;

关键词：

PROTEIN-DETECTION; AU NANOPARTICLES; AMPLIFICATION; SENSOR; ASSAY;

D O I：

10.1016/j.ab.2011.01.032

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：75 / 77

页数：3

共 15 条

[1]

Thrombin and prothrombin are expressed by neurons and glial cells and accumulate in neurofibrillary tangles in Alzheimer disease brain [J].

Arai, T ;

Miklossy, J ;

Klegeris, A ;

Guo, JP ;

McGeer, PL .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2006, 65 (01) :19-25

[2]

Aptamer-Based SERRS Sensor for Thrombin Detection [J].

Cho, Hansang ;

Baker, Brian R. ;

Wachsmann-Hogiu, Sebastian ;

Pagba, Cynthia V. ;

Laurence, Ted A. ;

Lane, Stephen M. ;

Lee, Luke P. ;

Tok, Jeffrey B. -H. .

NANO LETTERS, 2008, 8 (12) :4386-4390

[3]

Impedimetric Aptasensor with Femtomolar Sensitivity Based on the Enlargement of Surface-Charged Gold Nanoparticles [J].

Deng, Chunyan ;

Chen, Jinhua ;

Nie, Zhou ;

Wang, Mengdong ;

Chu, Xiaochen ;

Chen, Xiaoli ;

Xiao, Xilin ;

Lei, Chunyang ;

Yao, Shouzhuo .

ANALYTICAL CHEMISTRY, 2009, 81 (02) :739-745

[4]

Protein detection via direct enzymatic amplification of short DNA aptamers [J].

Fischer, Nicholas O. ;

Tarasow, Theodore M. ;

Tok, Jeffrey B. -H. .

ANALYTICAL BIOCHEMISTRY, 2008, 373 (01) :121-128

[5]

Ultrasensitive electrochemical detection of proteins by amplification of aptamer-nanoparticle bio bar codes [J].

He, Pingli ;

Shen, Li ;

Cao, Yunhe ;

Lia, Defa .

ANALYTICAL CHEMISTRY, 2007, 79 (21) :8024-8029

[6]

Effect of oligodeoxynucleotide thrombin aptamer on thrombin inhibition by heparin cofactor II and antithrombin [J].

Holland, CA ;

Henry, AT ;

Whinna, HC ;

Church, FC .

FEBS LETTERS, 2000, 484 (02) :87-91

[7]

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor [J].

Huang, Po-Jung Jimmy ;

Liu, Juewen .

ANALYTICAL CHEMISTRY, 2010, 82 (10) :4020-4026

[8]

Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin [J].

Pavlov, V ;

Xiao, Y ;

Shlyahovsky, B ;

Willner, I .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (38) :11768-11769

[9]

Clinical development of bivalirudin (Angiox®):: rationale for thrombin-specific anticoagulation in percutaneous coronary intervention and acute coronary syndromes [J].

Serruys, PW ;

Vranckx, P ;

Allikmets, K .

INTERNATIONAL JOURNAL OF CLINICAL PRACTICE, 2006, 60 (03) :344-350

[10]

On-Chip Aptamer-Based Sandwich Assay for Thrombin Detection Employing Magnetic Beads and Quantum Dots [J].

Tennico, Yolanda H. ;

Hutanu, Daniela ;

Koesdjojo, Myra T. ;

Bartel, Cheryl Moody ;

Remcho, Vincent T. .

ANALYTICAL CHEMISTRY, 2010, 82 (13) :5591-5597

← 1 2 →