Cloning and mapping of resistance gene homologues in melon

被引:30
作者
Garcia-Mas, J
van Leeuwen, H
Monfort, A
de Vicente, MC
Puigdomènech, P
Arús, P
机构
[1] IRTA, Dept Genet Vegetal, Barcelona 08348, Spain
[2] CSIC, CID, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain
关键词
disease resistance; RGH; mapping; Cucumis melo;
D O I
10.1016/S0168-9452(01)00403-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Four families of resistance gene homologues containing the nucleotide binding site (NBS) motif were obtained in Cucumis melo using degenerate primers designed from two conserved regions of known disease resistance genes. The four homologues had high levels of amino acid identity to NBS-containing resistance genes found in other species. Three of these homologues (MRGH4, MRGH21 and MRGH63) were placed in the genetic map of melon. The RFLP detected with MRGH21 was located on the distal part of Linkage Group 7. Homologues MRGH63 and MRGH4 detected RFLPs that mapped to Linkage Group 4. The map positions of these loci relative to those of resistance genes mapped in melon and the potential use of these loci in marker-assisted selection and map-based cloning strategies is discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:165 / 172
页数:8
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