Nervous and nonnervous cell transduction by recombinant adenoviruses that inducibly express the human PrP

被引：6

作者：

Arrabal, S

Touchard, M

Mouthon, F

Klonjkowski, B

Deslys, JP

Dormont, D

Eloit, M ^{[1
]}

机构：

[1] Ecole Natl Vet Alfort, URA INRA, F-94704 Maisons Alfort, France

[2] CEA, Serv Neurovirol, DSV, DRM,CRSSA,EPHE, F-92265 Fontenay Aux Roses, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 285卷 / 03期

关键词：

D O I：

10.1006/bbrc.2001.5208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study of the prion protein (PrP) physiological functions or its specific role in transmissible spongiform encephalopathies (TSE) requires new tools, particularly those able to induce PrP overexpression in a large range of cells, in vivo as well as in vitro. Here we describe the construction of two recombinant adenoviruses encoding the human PrP either with a valine at position 129 (AdTRVal) or a methionine (AdTRMet). Both genes were put under the control of the tetracycline-responsive promoter, allowing tight regulation of PrP expression. AdTRVal and AdTRMet induced high expression of the human PrP in CHO-KI cells and in organotypic brain slices in culture. The proteins expressed from these viruses exhibited a glycosylphosphatidyl inositol (GPI) anchor, proper glycosylation and sensitivity to proteinase K digestion. AdTRVal and AdTRMet will allow future studies on the human PrP and on the role of the codon 129 polyphormism in human TSE. (C) 2001 Academic Press.

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页码：623 / 632

页数：10

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