A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells

被引：37

作者：

Cockrell, Diane C. ^{[1
]}

Beare, Paul A. ^{[1
]}

Fischer, Elizabeth R. ^{[2
]}

Howe, Dale ^{[1
]}

Heinzen, Robert. A. ^{[1
]}

机构：

[1] Natl Inst Hlth, Natl Inst Allergy & Infect Dis, Rocky Mt Labs, Intracellular Parasites Lab,Coxiella Pathogenesis, Hamilton, MT 59840 USA

[2] Natl Inst Hlth, Natl Inst Allergy & Infect Dis, Rocky Mt Labs, Res Technol Branch,Microscopy Unit,Res Technol Br, Hamilton, MT 59840 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 72卷 / 03期

关键词：

digitonin; Coxiella; Q fever; cell lysis; purification; vacuole; infectivity; sonication; intracellular parasite;

D O I：

10.1016/j.mimet.2007.12.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication. Published by Elsevier B.V.

引用

页码：321 / 325

页数：5

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