The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice

被引:270
作者
Nonomura, KI
Miyoshi, K
Eiguchi, M
Suzuki, T
Miyao, A
Hirochika, H
Kurata, N
机构
[1] Natl Inst Genet, Expt Farm Plant Genet Lab, Mishima, Shizuoka 4118540, Japan
[2] Natl Inst Agrobiol Sci, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan
[3] Grad Univ Adv Studies, Dept Life Sci, Mishima, Shizuoka 4118540, Japan
关键词
D O I
10.1105/tpc.012401
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The function of the novel gene MSP1 (MULTIPLE SPOROCYTE), which controls early sporogenic development, was elucidated by characterizing a retrotransposon-tagged mutation of rice. The MSP1 gene encoded a Leu-rich repeat receptor-like protein kinase. The msp1 mutation gave rise to an excessive number of both male and female sporocytes. In addition, the formation of anther wall layers was disordered and the tapetum layer was lost completely. Although the mutation never affected homologous chromosome pairing and chiasma maintenance, the development of pollen mother cells was arrested at various stages of meiotic prophase I, which resulted in complete male sterility. Meanwhile, plural megaspore mother cells in a mutant ovule generated several megaspores, underwent gametogenesis, and produced germinable seeds when fertilized with wild-type pollen despite disorganized female gametophytes. In situ expression of MSP1 was detected in surrounding cells of male and female sporocytes and some flower tissues, but never in the sporocytes themselves. These results suggest that the MSP1 product plays crucial roles in restricting the number of cells entering into male and female sporogenesis and in initiating anther wall formation in rice.
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收藏
页码:1728 / 1739
页数:12
相关论文
共 50 条
[31]   Switch (swi1), an Arabidopsis thaliana mutant affected in the female meiotic switch [J].
Motamayor, JC ;
Vezon, D ;
Bajon, C ;
Sauvanet, A ;
Grandjean, O ;
Marchand, M ;
Bechtold, N ;
Pelletier, G ;
Horlow, C .
SEXUAL PLANT REPRODUCTION, 2000, 12 (04) :209-218
[32]   A KNOWLEDGE BASE FOR PREDICTING PROTEIN LOCALIZATION SITES IN EUKARYOTIC CELLS [J].
NAKAI, K ;
KANEHISA, M .
GENOMICS, 1992, 14 (04) :897-911
[33]   GENETIC MOSAICS AND CELL LINEAGE ANALYSIS IN PLANTS [J].
POETHIG, S .
TRENDS IN GENETICS, 1989, 5 (08) :273-277
[34]   ANTHER AND POLLEN DEVELOPMENT IN RICE (ORYZA-SATIVA) [J].
RAGHAVAN, V .
AMERICAN JOURNAL OF BOTANY, 1988, 75 (02) :183-196
[35]   FINE-STRUCTURE OF MEGAGAMETOPHYTE DEVELOPMENT IN ZEA-MAYS [J].
RUSSELL, SD .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1979, 57 (10) :1093-1110
[36]  
Sambrook J., 1989, MOL CLONING
[37]   Isolation and characterization of a rice homeobox gene, OSH15 [J].
Sato, Y ;
Sentoku, N ;
Nagato, Y ;
Matsuoka, M .
PLANT MOLECULAR BIOLOGY, 1998, 38 (06) :983-998
[38]   Molecular analysis of NOZZLE, a gene involved in pattern formation and early sporogenesis during sex organ development in Arabidopsis thaliana [J].
Schiefthaler, U ;
Balasubramanian, S ;
Sieber, P ;
Chevalier, D ;
Wisman, E ;
Schneitz, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11664-11669
[39]   Regional expression of the rice KN1-type homeobox gene family during embryo, shoot, and flower development [J].
Sentoku, N ;
Sato, Y ;
Kurata, N ;
Ito, Y ;
Kitano, H ;
Matsuoka, M .
PLANT CELL, 1999, 11 (09) :1651-1663
[40]  
Sheridan WF, 1999, GENETICS, V153, P933