Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs

被引：59

作者：

Tomoyasu, Akihiro

Higashio, Kanji

Kanomata, Kazuhiro

Goto, Masaaki

Kodaira, Kunihiko

Serizawa, Hiroko

Suda, Tatsuo

Nakamura, Atsushi

Nojima, Junya

Fukuda, Toru

Katagiri, Takenobu

机构：

[1] Saitama Med Univ, Res Ctr Genom Med, Div Pathophysiol, Hidaka, Saitama 3501241, Japan

[2] Metabolome Pharmaceut Inc, Meguro Ku, Tokyo 1530065, Japan

[3] Chugai Pharmaceut Co Ltd, Fuji Gotemba Res Labs, Genome Antibody Prod Res Div, Shizuoka 4128513, Japan

[4] Chugai Pharmaceut Co Ltd, Drug Engn Div, Kita Ku, Tokyo 1158543, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 361卷 / 01期

关键词：

bone morphogenetic proteins; platelet-rich plasma; osteoblasts; scaffolds;

D O I：

10.1016/j.bbrc.2007.06.142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelet-rich plasma (PRP) is clinically used as an autologous blood product to stimulate bone formation in vivo. In the present study, we examined the effects of PRP on proliferation and osteoblast differentiation in vitro in the presence of bone morphogenetic proteins (BMPs). PRP and its soluble fraction stimulated osteoblastic differentiation of myoblasts and osteoblastic cells in the presence of BMP-2, BMP-4, BMP-6 or BMP-7. The soluble PRP fraction stimulated osteoblastic differentiation in 3D cultures using scaffolds made of collagen or hydroxyapatite. Moreover, heparin-binding fractions obtained from serum also stimulated osteoblastic differentiation in the presence of BMP-4. These results suggested that platelets contain not only growth factors for proliferation but also novel potentiator(s) for BMP-dependent osteoblastic differentiation. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：62 / 67

页数：6

共 23 条

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