Hepatocyte spheroids in polyurethane foams: Functional analysis and application for a hybrid artificial liver

被引:88
作者
Ijima, H [1 ]
Matsushita, T [1 ]
Nakazawa, K [1 ]
Fujii, Y [1 ]
Funatsu, K [1 ]
机构
[1] Kyushu Univ, Grad Sch Engn, Dept Chem Syst & Engn, Chem Engn Grp,Higashi Ku, Fukuoka 8128581, Japan
来源
TISSUE ENGINEERING | 1998年 / 4卷 / 02期
关键词
D O I
10.1089/ten.1998.4.213
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Spherical multicellular aggregates (spheroids) of adult rat hepatocytes were spontaneously formed in the pores of polyurethane foam (PUF) as a culture substratum. Highly differentiated functions of hepatocytes, that is, albumin secretion, urea synthesis, and drug metabolism, were maintained in the spheroid culture using PUF. These spheroids were partly attached and immobilized in the pores of PUF. Then, we designed a PUF/spheroids packed-bed module as a hybrid artificial liver and developed an artificial liver support system, including an extracorporeal circulation line for recovery of a hepatic failure rat induced by D-galactosamine. Consequently, increases in blood ammonia concentrations in hepatic failure rats were suppressed below the hepatic coma level by the artificial liver support system, and 80% of these rats recovered from hepatic failure. From these results, it was suggested that hepatocytes/spheroid culture seemed to provide a promising culture system as a hybrid artificial liver.
引用
收藏
页码:213 / 226
页数:14
相关论文
共 21 条
[1]   LONG-TERM INVITRO FUNCTION OF ADULT HEPATOCYTES IN A COLLAGEN SANDWICH CONFIGURATION [J].
DUNN, JCY ;
TOMPKINS, RG ;
YARMUSH, ML .
BIOTECHNOLOGY PROGRESS, 1991, 7 (03) :237-245
[2]  
FUNATSU K, 1994, ARTIF ORGAN TODAY, V3, P253
[3]   THE USE OF FLUORESCEIN DIACETATE AND ETHIDIUM-BROMIDE AS A VIABILITY STAIN FOR ISOLATED ISLETS OF LANGERHANS [J].
GRAY, DWR ;
MORRIS, PJ .
STAIN TECHNOLOGY, 1987, 62 (06) :379-381
[4]  
Ijima H., 1994, Japanese Journal of Artificial Organs, V23, P463
[5]  
KASAI S, 1990, Japanese Journal of Artificial Organs, V19, P1545
[6]  
KAWAGUCHI M, 1989, JPN J ARTIF ORGANS, V18, P1269
[7]   CONTINUED HIGH ALBUMIN PRODUCTION BY MULTICELLULAR SPHEROIDS OF ADULT-RAT HEPATOCYTES FORMED IN THE PRESENCE OF LIVER-DERIVED PROTEOGLYCANS [J].
KOIDE, N ;
SHINJI, T ;
TANABE, T ;
ASANO, K ;
KAWAGUCHI, M ;
SAKAGUCHI, K ;
KOIDE, Y ;
MORI, M ;
TSUJI, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (01) :385-391
[8]   FORMATION OF MULTICELLULAR SPHEROIDS COMPOSED OF ADULT-RAT HEPATOCYTES IN DISHES WITH POSITIVELY CHARGED SURFACES AND UNDER OTHER NONADHERENT ENVIRONMENTS [J].
KOIDE, N ;
SAKAGUCHI, K ;
KOIDE, Y ;
ASANO, K ;
KAWAGUCHI, M ;
MATSUSHIMA, H ;
TAKENAMI, T ;
SHINJI, T ;
MORI, M ;
TSUJI, T .
EXPERIMENTAL CELL RESEARCH, 1990, 186 (02) :227-235
[9]   ANCHORAGE-DEPENDENT MAMMALIAN-CELL CULTURE USING POLYURETHANE FOAM AS A NEW SUBSTRATUM FOR CELL ATTACHMENT [J].
MATSUSHITA, T ;
KETAYAMA, M ;
KAMIHATA, K ;
FUNATSU, K .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 33 (03) :287-290
[10]  
MATSUSHITA T, 1991, APPL MICROBIOL BIOT, V36, P324