ORTHOGONAL GENE EXPRESSION IN ESCHERICHIA COLI

被引：3

作者：

An, Wenlin ^{[1
]}

Chin, Jason W. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

METHODS IN ENZYMOLOGY, VOL 497: SYNTHETIC BIOLOGY, METHODS FOR PART/DEVICE CHARACTERIZATION AND CHASSIS ENGINEERING, PT A | 2011年 / 497卷

基金：

英国医学研究理事会;

关键词：

RIBONUCLEIC-ACID POLYMERASE; THERMUS-THERMOPHILUS HB8; RIBOSOMAL-RNA OPERON; SYNTHETIC BIOLOGY; NETWORKS; TRANSCRIPTION; TRANSLATION; EFFICIENCY; MECHANISM; CIRCUITS;

D O I：

10.1016/B978-0-12-385075-1.00005-6

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Here, we describe a route orthogonal gene expression which combines orthogonal transcription and translation using library-based selections. We show how orthogonal gene expression can be used to create a minimal orthogonal ribosome and describe how to create orthogonal transcription translation feed forward loops that introduce tailored information processing delays into gene expression.

引用

收藏

页码：115 / 134

页数：20

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