Inhibition of Class-3 aldehyde dehydrogenase and cell growth by restored lipid peroxidation in hepatoma cell lines

被引:28
作者
Canuto, RA [1 ]
Muzio, G
Ferro, M
Maggiora, M
Federa, R
Bassi, AM
Lindahl, R
Dianzani, MU
机构
[1] Univ Turin, Osped S Luigi, Dipartimento Sci Clin & Biol, I-10043 Turin, Italy
[2] Univ Genoa, Ist Patol Gen, I-16132 Genoa, Italy
[3] Univ S Dakota, Dept Biochem & Mol Biol, Vermillion, SD 57069 USA
关键词
lipid peroxidation; hepatoma; aldehyde dehydrogenase; arachidonic acid; ascorbate/iron sulphate; MDA production; free radical;
D O I
10.1016/S0891-5849(98)00206-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hepatoma cells have a below-normal content of polyunsaturated fatty acids; this reduces lipid peroxidation and the production of cytotoxic and cytostatic aldehydes within the cells. In proportion to the degree of deviation, hepatoma cells also show an increase in the activity of Class-3 aldehyde dehydrogenase, an enzyme important in the metabolism of lipid peroxidation products and also in that of several drugs. When hepatoma cells with different degrees of deviation were enriched with arachidonic acid and stimulated to peroxidize by ascorbate/iron sulphate, their growth rate was reduced in proportion to the quantity of aldehydes produced and to the activity of aldehyde dehydrogenase. Therefore, 7777 cells, less deviated and with low Class-3 aldehyde dehydrogenase activity, were more susceptible to lipid peroxidation products than JM2 cells. It is noteworthy that repeated treatments with prooxidant also caused a decrease in mRNA and activity of Class-3 aldehyde dehydrogenase, contributing to the decreased growth and viability. Thus, Class-3 aldehyde dehydrogenase could be considered relevant for the growth of hepatoma cells, since it defends them against cell growth inhibiting aldehydes derived from Lipid peroxidation. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:333 / 340
页数:8
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